Yıl 2018, Cilt 11, Sayı 3, Sayfalar 238 - 244 2018-09-01

Afyonkarahisar İlinde Ruminant Abort Vakalarında Pestivirusların (BDV ve BVDV) Rolleri
The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province

Murat ŞEVİK [1]

71 75

Pestiviruslar ruminantlarda abortlara neden olan önemli viral ajanlardır. Bu çalışmada, ruminant abort vakalarında Border Disease Virus (BDV) ve Bovine Viral Diarrhoea Virus (BVDV)’un rolleri araştırılmıştır. Abort olmuş fötus doku örnekleri 101 hayvandan (74’ü koyun fötusu, 27’si sığır fötus), her biri epidemiyolojik olarak farklı çiftliklerden, Ocak 2016 ve Aralık 2017 ayları arasında Afyonkarahisar ilinden toplanmıştır. BDV ve BVDV RNA’sının tespiti için tek adımlı real-time dubleks RT-PCR yöntemi kullanılmıştır. Sahadan izole edilen pestivirus’ların genetik karakterizasyonu 5' translate olmayan bölge sonunun (5' UTR) sekansı ile gerçekleştirilmiştir. BDV RNA’sı, 74 aborte koyun fötusunun 9 (%12.16)’unda, BVDV RNA’sı ise 27 sığır fötusunun 6 (%22.2)’sında tespit edilmiştir.  5' UTR bölgesinin filogenetik analizi bu çalışmada izole edilen BDV izolatlarının BDV-7 genotipine, BVDV izolatlarının ise BVDV-1 genotipine ait olduğunu göstermiştir. Bu çalışmanın sonuçları, pestivirus enfeksiyonlarının, Afyonkarahisar ilindeki ruminant abort vakalarında önemli rol oynadığını göstermektedir.

Pestiviruses are important viral agents that can cause abortion in ruminants. In this study, roles of Border Disease Virus (BDV) and Bovine Viral Diarrhoea Virus (BVDV) were investigated in ruminant abortion cases. Aborted foetal tissue samples were collected from 101 animals (74 sheep foetuses and 27 bovine foetuses), each from epidemiologically different farms, during the months of January 2016 and December 2017 in the Afyonkarahisar Province. One step real-time duplex RT-PCR was used for the detection of BDV and BVDV RNA. Genetic characterization of the field isolates of pestiviruses was conducted by sequencing 5' untranslated region (5' UTR). BDV RNA was detected in 9 (12.16%) of the 74 aborted sheep foetuses, whereas BVDV RNA was detected in 6 (22.2%) of the 27 bovine foetuses. Phylogenetic analysis based on the 5' UTR region indicated that BDV isolates in the present study belong to BDV-7 genotype whereas BVDV isolates belong to BVDV-1 genotype. The results of this study showed that pestivirus infections play important role in ruminant abortion cases in Afyonkarahisar province.

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Yazar: Murat ŞEVİK (Sorumlu Yazar)
Kurum: Mustafa Kemal University, Veterinary Faculty
Ülke: Turkey


Bibtex @araştırma makalesi { kvj418451, journal = {Kocatepe Veteriner Dergisi}, issn = {1308-1594}, eissn = {2147-6853}, address = {Afyon Kocatepe Üniversitesi}, year = {2018}, volume = {11}, pages = {238 - 244}, doi = {10.30607/kvj.418451}, title = {The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province}, key = {cite}, author = {ŞEVİK, Murat} }
APA ŞEVİK, M . (2018). The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province. Kocatepe Veteriner Dergisi, 11 (3), 238-244. DOI: 10.30607/kvj.418451
MLA ŞEVİK, M . "The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province". Kocatepe Veteriner Dergisi 11 (2018): 238-244 <http://dergipark.gov.tr/kvj/issue/37070/418451>
Chicago ŞEVİK, M . "The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province". Kocatepe Veteriner Dergisi 11 (2018): 238-244
RIS TY - JOUR T1 - The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province AU - Murat ŞEVİK Y1 - 2018 PY - 2018 N1 - doi: 10.30607/kvj.418451 DO - 10.30607/kvj.418451 T2 - Kocatepe Veteriner Dergisi JF - Journal JO - JOR SP - 238 EP - 244 VL - 11 IS - 3 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.418451 UR - http://dx.doi.org/10.30607/kvj.418451 Y2 - 2018 ER -
EndNote %0 Kocatepe Veteriner Dergisi The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province %A Murat ŞEVİK %T The Role of Pestiviruses (BDV and BVDV) in Ruminant Abortion Cases in the Afyonkarahisar Province %D 2018 %J Kocatepe Veteriner Dergisi %P 1308-1594-2147-6853 %V 11 %N 3 %R doi: 10.30607/kvj.418451 %U 10.30607/kvj.418451
ISNAD ŞEVİK, Murat . "Afyonkarahisar İlinde Ruminant Abort Vakalarında Pestivirusların (BDV ve BVDV) Rolleri". Kocatepe Veteriner Dergisi 11 / 3 (Eylül 2018): 238-244. http://dx.doi.org/10.30607/kvj.418451