Yıl 2018, Cilt 9, Sayı 2, Sayfalar 129 - 135 2018-04-25

Drosophila’da Aspartam Tarafından Oluşturulan Mitokondrial DNA Hasarı
Aspartame-Induced Mitochondrial DNA Damage in Drosophila

Ayşe Gül MUTLU [1] , Nesibe KÜRKLÜ [2]

81 191

Yapay tatlandırıcılar çok çeşitli yiyecek, içecek, ilaç ve hijyen ürünlerine eklenir. Bu ürünlerin herhangi birinin toksik etkilerinin olması, toplumdaki birçok insan tarafından kullanıldığı için, halk sağlığı açısından risk oluşturabilir. Bazı yapay tatlandırıcıların nüklear DNA üzerindeki etkileri araştırılmış, ancak bu maddelerin mtDNA üzerindeki etkileri hakkında herhangi bir çalışmaya literatürde rastlanmamıştır. Mitokondrial genomdaki tüm bölgeler ifade edildiğinden, mtDNA’daki mutasyonlar, nDNA’daki delesyonlardan potansiyel olarak daha önemli olabilir. Ayrıca, primer insan kanserlerinde somatik mtDNA mutasyonları gittikçe artan bir şekilde gözlenmektedir. Bu çalışmanın amacı, bazı yapay tatlandırıcıların mtDNA hasarı ve kopya sayısına etkilerinin Drosophila melanogaster’de saptanmasıdır. Bu amaçla kantitatif PCR yöntemi kullanılmıştır. Sonuçlarımıza göre, Aspartam istatistiksel olarak anlamlı derecede mtDNA hasarı oluşturmuştur. Sakkarin + Siklamat, Sakkarin, Asesulfam K ve Sukraloz uygulama gruplarında ise mtDNA hasarı saptanmamıştır. Bu sonuçlar, Aspartamın insanlar üzerinde yapabileceği olası etkilerinin dikkatle saptanması gerektiğini göstermektedir.

Artificial sweeteners are added to many products sold in the market today, such as food, beverages, medicines, hygiene products. The possible toxic effects of any of these products, because they are used by many people in the community, can pose a risk for public health. DNA mutations generated by some artificial sweeteners have been investigated by some researchers but there is no information in the literature about the effects of these substances on mtDNA. mtDNA damage potentially is more important than deletions in nDNA because the whole mitochondrial genome is expressed without intron regions. Also, somatic mtDNA mutations have been increasingly observed in primary human cancers. The aim of the current study, detection of the effects of some artificial sweeteners on mtDNA damage and copy number in Drosophila melanogaster. The Quantitative PCR method was used for this purpose. According to our results, Aspartame created statistically significant mtDNA damage. There was no mtDNA damage in Saccharine+Cyclamate, Saccharine, Aceculfam K and Sucralose application groups. These results indicate that the effects of aspartame in human should carefully detect.

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Birincil Dil en
Konular Mühendislik
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Ayşe Gül MUTLU (Sorumlu Yazar)
Kurum: MEHMET AKİF ERSOY ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ
Ülke: Turkey


Yazar: Nesibe KÜRKLÜ

Bibtex @araştırma makalesi { makufebed377982, journal = {Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi}, issn = {}, eissn = {1309-2243}, address = {Mehmet Akif Ersoy Üniversitesi}, year = {2018}, volume = {9}, pages = {129 - 135}, doi = {10.29048/makufebed.377982}, title = {Aspartame-Induced Mitochondrial DNA Damage in Drosophila}, key = {cite}, author = {KÜRKLÜ, Nesibe and MUTLU, Ayşe Gül} }
APA MUTLU, A , KÜRKLÜ, N . (2018). Aspartame-Induced Mitochondrial DNA Damage in Drosophila. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9 (2), 129-135. DOI: 10.29048/makufebed.377982
MLA MUTLU, A , KÜRKLÜ, N . "Aspartame-Induced Mitochondrial DNA Damage in Drosophila". Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi 9 (2018): 129-135 <http://dergipark.gov.tr/makufebed/issue/35999/377982>
Chicago MUTLU, A , KÜRKLÜ, N . "Aspartame-Induced Mitochondrial DNA Damage in Drosophila". Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi 9 (2018): 129-135
RIS TY - JOUR T1 - Aspartame-Induced Mitochondrial DNA Damage in Drosophila AU - Ayşe Gül MUTLU , Nesibe KÜRKLÜ Y1 - 2018 PY - 2018 N1 - doi: 10.29048/makufebed.377982 DO - 10.29048/makufebed.377982 T2 - Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi JF - Journal JO - JOR SP - 129 EP - 135 VL - 9 IS - 2 SN - -1309-2243 M3 - doi: 10.29048/makufebed.377982 UR - http://dx.doi.org/10.29048/makufebed.377982 Y2 - 2018 ER -
EndNote %0 Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi Aspartame-Induced Mitochondrial DNA Damage in Drosophila %A Ayşe Gül MUTLU , Nesibe KÜRKLÜ %T Aspartame-Induced Mitochondrial DNA Damage in Drosophila %D 2018 %J Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi %P -1309-2243 %V 9 %N 2 %R doi: 10.29048/makufebed.377982 %U 10.29048/makufebed.377982
ISNAD MUTLU, Ayşe Gül , KÜRKLÜ, Nesibe . "Drosophila’da Aspartam Tarafından Oluşturulan Mitokondrial DNA Hasarı". Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi 9 / 2 (Nisan 2018): 129-135. http://dx.doi.org/10.29048/makufebed.377982