Yıl 2017, Cilt 1, Sayı 2, Sayfalar 43 - 48 2017-12-31

Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture

Atilla Günay [1] , Ş. Şebnem Ellialtıoğlu [2] , selma Herdem [3] , Damla Turan Büyükdinç [4]

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Callus and cell suspension cultures provide significant advantages to reveal somaclonal variation in plant breeding studies. When mutagen treatments are integrated, tissue culture techniques can be used to increase the genetic variation to higher frequencies. Callus tissues may provide advantages for mutation applications in breeding studies of carrot to be performed in vitro. For this, optimizing the plant regeneration system from callus and cell suspensions is the first step necessary. Carrot is a plant species which has quite high ability of somatic embryogenesis. In this research aimed at establishing a basis for breeding purposes, tissue fractions containing cambium from the Nantes variety of ripe carrot roots were cultured in MS medium containing different doses of 2,4-D on aseptic conditions. The formation of shoots was observed from callus tissues transplanted to 2,4-D and Kinetin combinations. Callus tissue obtained from medium containing 0.5 mg/L 2,4-D was used to obtain cell suspensions. Two different treatment procedures were applied for the cell suspension. After subcultures containing 1 mg/L 2,4-D, cell clusters are formed in callus suspensions transferred to hormone-free liquid or agar media. Somatic embryogenesis was obtained in agar supplemented media. The method for callus regeneration obtained from the root cambium cells has been successfully applied.

Carrot,callus,cell suspension,2,4-D,kinetin,organogenesis
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Yazar: Atilla Günay
E-posta: agunay@ankara.edu.tr

Yazar: Ş. Şebnem Ellialtıoğlu
E-posta: sebnemellialti@gmail.com

Yazar: selma Herdem
E-posta: selmaherdem@gmail.com

Yazar: Damla Turan Büyükdinç (Sorumlu Yazar)
E-posta: damla.turan@erdogan.edu.tr

Bibtex @araştırma makalesi { molbiotech393208, journal = {Journal of Molecular Biology and Biotechnology}, issn = {}, address = {Nobel Bilim ve Araştırma Merkezi Limited}, year = {2017}, volume = {1}, pages = {43 - 48}, doi = {}, title = {Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture}, key = {cite}, author = {Ellialtıoğlu, Ş. Şebnem and Herdem, selma and Turan Büyükdinç, Damla and Günay, Atilla} }
APA Günay, A , Ellialtıoğlu, Ş , Herdem, s , Turan Büyükdinç, D . (2017). Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture. Journal of Molecular Biology and Biotechnology, 1 (2), 43-48. Retrieved from http://dergipark.gov.tr/molbiotech/issue/35401/393208
MLA Günay, A , Ellialtıoğlu, Ş , Herdem, s , Turan Büyükdinç, D . "Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture". Journal of Molecular Biology and Biotechnology 1 (2017): 43-48 <http://dergipark.gov.tr/molbiotech/issue/35401/393208>
Chicago Günay, A , Ellialtıoğlu, Ş , Herdem, s , Turan Büyükdinç, D . "Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture". Journal of Molecular Biology and Biotechnology 1 (2017): 43-48
RIS TY - JOUR T1 - Somatic Embryogenesis from Root Cambium Tissue of Carrots (Daucus carota L.) by Using Callus and Cell Suspension Culture AU - Atilla Günay , Ş. Şebnem Ellialtıoğlu , selma Herdem , Damla Turan Büyükdinç Y1 - 2017 PY - 2017 N1 - DO - T2 - Journal of Molecular Biology and Biotechnology JF - Journal JO - JOR SP - 43 EP - 48 VL - 1 IS - 2 SN - -1308-6561 M3 - UR - Y2 - 2018 ER -
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