Yıl 2017, Cilt 3, Sayı 3, Sayfalar 33 - 38 2017-09-30

Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate

Nur Agirman [1] , Ahmet Kadri Cetin [2]

229 353

Objective: In this study effects of nitrogen limitation on growth, total lipid and protein amount in Scenedesmus acutus.

Material and Methods: The microalgal strain of Scenedesmus acutus used in this study was isolated from the Keban Reservoir in Eastern Anatolia, Turkey was grown in Jaworsky’s medium. Algal cells were calculated by measuring the optical density at 680 nm using a visible density spectrophotometer. The total lipid content was determined using the Bligh and Dyer method. The total protein content was determined by the Lowry method.

Results: The results show that there is an inverse relationship between cellular growth, lipid amount and nitrogen concentration. S. acutus survived all the nitrogen concentrations tested and increases were observed in the amount of its cellular lipid. It was determined that there was enough nitrogen in the nitrogen-limited media to support protein synthesis and cell growth of S. acutus and that the amount of lipid in the 50% nitrogen-limited media was 19.48% higher than that in the control group.

Conclusion: S. acutus survived all the nitrogen concentrations tested and increases were observed in the amount of its cellular lipid. Significant increase in the amount of lipid in Scenedesmus acutus subjected to nitrogen stress suggests the idea that the microalga in question can be one of the potential organisms that can be used to obtain biofuel.


Scenedesmus acutus, nitrogen limitation, protein, lipid, biofuel
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Konular Yaşam Bilimleri
Dergi Bölümü Original Articles
Yazarlar

Yazar: Nur Agirman
Kurum: Firat University, Faculty of Science, Dept. of Biology, Elazig
Ülke: Turkey


Yazar: Ahmet Kadri Cetin
Kurum: Firat University, Faculty of Science, Dept. of Biology, Elazig
Ülke: Turkey


Bibtex @araştırma makalesi { nsd322614, journal = {Natural Science and Discovery}, issn = {2149-6307}, eissn = {2149-6307}, address = {Lycia Press Inc.}, year = {2017}, volume = {3}, pages = {33 - 38}, doi = {10.20863/nsd.322614}, title = {Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate}, key = {cite}, author = {Agirman, Nur and Cetin, Ahmet} }
APA Agirman, N , Cetin, A . (2017). Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate. Natural Science and Discovery, 3 (3), 33-38. DOI: 10.20863/nsd.322614
MLA Agirman, N , Cetin, A . "Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate". Natural Science and Discovery 3 (2017): 33-38 <http://dergipark.gov.tr/nsd/issue/30487/322614>
Chicago Agirman, N , Cetin, A . "Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate". Natural Science and Discovery 3 (2017): 33-38
RIS TY - JOUR T1 - Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate AU - Nur Agirman , Ahmet Kadri Cetin Y1 - 2017 PY - 2017 N1 - doi: 10.20863/nsd.322614 DO - 10.20863/nsd.322614 T2 - Natural Science and Discovery JF - Journal JO - JOR SP - 33 EP - 38 VL - 3 IS - 3 SN - 2149-6307-2149-6307 M3 - doi: 10.20863/nsd.322614 UR - http://dx.doi.org/10.20863/nsd.322614 Y2 - 2017 ER -
EndNote %0 Natural Science and Discovery Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate %A Nur Agirman , Ahmet Kadri Cetin %T Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate %D 2017 %J Natural Science and Discovery %P 2149-6307-2149-6307 %V 3 %N 3 %R doi: 10.20863/nsd.322614 %U 10.20863/nsd.322614
ISNAD Agirman, Nur , Cetin, Ahmet . "Effect of nitrogen limitation on growth, total lipid accumulation and protein amount in Scenedesmus acutus as biofuel reactor candidate". Natural Science and Discovery 3 / 3 (Eylül 2017): 33-38. http://dx.doi.org/10.20863/nsd.322614