Yıl 2017, Cilt 3, Sayı 3, Sayfalar 39 - 43 2017-09-30

Karyotype analysis of Common Cocklebur (Xanthium strumarium L.)

Gulden Dogan [1] , Yasar Kiran [2]

229 393

Objective: The purpose of this study is to determine the mitotic chromosome number, morphometric parameters, and karyotypes of Xanthium strumarium and make contributions to other multidisciplinary studies on the genus.

Material and Methods: The seeds were germinated on moist filter paper in Petri dishes at 25°C. Actively growing root tips were pre-treated with aqueous paradichlorobenzene for 4h at room temperature. Then, the root tips were fixed with acetic alcohol (1:3 glacial acetic acid–absolute ethanol) for at least 24 h at 4°C, hydrolysed in 1 N HCl at 60°C for 3 min, then rinsed in tap water for 3–5 min. Finally, they were stained in Feulgen for 1 h and mounted in 45% acetic acid. Digital microphotographs from at least five well-spread metaphase plates were taken using an Olympus BX51 microscope and were recorded with an Olympus Camedia C-4000 digital camera.

Results: The chromosome number is determined as 2n = 36 for this taxon. The karyotype consists of 16 median region (m) and 2 submedian region (sm) chromosomes. The metaphase chromosome length ranges from 2.30 to 4.03 μm, longest to shortest chromosome ratio is 1.7:1.1, total karyotype length (TKL) 54.76 m and the karyotype symmetry is type 1A.

Conclusion: The results of this study showed that the chromosome number of Xanthium strumarium is 2n=36. Satellites were not observed in the karyotype of this species. Identifying the chromosome number of this species in this study provides a base for biosystematic studies.

Chromosome number, Xanthium
  • 1. Weaver SE, Lechowich MJ. The biology of Canadian weeds. 56. Xanthium strumarium L. J Plant Sci. 1982; 63:211-225.
  • 2. Caius JF. Medicinal and poisonous plants of India. Pbl Sci Publ Jodhpur India. 1986; 375-376.
  • 3. Love D, Dansureau P. Biosystematic studies on Xanthium: taxonomic appraisal and ecological status. Can J Bot. 1959; 37:173-208. 4. Prain D. Bengal plants-II, Botanical Survey of India, Calcutta. Bishen Singh Mahendra Pal Singh, Dehradun, 1903; 607-608.
  • 5. Oudhia P, Dixit A. Weeds in Ambimkapur region (Madhya Pradesh) and their traditional use. Weed News. 1994; 1:19-21.
  • 6. Davis PH. Flora of Turkey and the East Aegean Island, Vol. 5. Edinburgh University Press: Edinburgh; 1975.
  • 7. Flora of the Ussr [In Russian]. Vol. 25. Izd. Akad. Nauk SSSR, Moscow, Leningrad. 1959; 521.
  • 8. Plant resources of Russia, Wild Flowering Plants, Their Quantitative Composition and Biological Activity [in Russian]. Vol. 5. Part 2. St. Petersburg, Moscow. 2013; 139-141.
  • 9. Makhlayuk VP. Medicinal Plants in Folk Medicine [in Russian], Povolzhskoe Kn. Izd. Saratov. 1991; 131-132.
  • 10. Ladynina EA. Wisdom of Herbs, Herbal Cures and Homeopathy [in Russian], AIF Print, Moscow. 2003; 216–217.
  • 11. Shrivastava KS, Krisnamurthy RS, Haksar CN. J Sci Ind Res Sect B. 1957; 16: 427.
  • 12. Chandel S, Bagai U, Vashishat N. Antiplasmodial activity of Xanthium strumarium against Plasmodium berghei-infected BALB/c mice. Parasitol Res. 2012; 110(3):1179-83.
  • 13. Gautam R, Saklani A, Jachak SM. Indian medicinal plants as a source of antimycobacterial agents. J Ethnopharmacol. 2007; 110(2):200-34.
  • 14. Ma YT, Huang MC, Hsu FL, Chang HF. Thiazinedione from Xanthium strumarium. Phytochemistry. 1998; 48(6):1083–1085.
  • 15. Yin MH, Kang DG, Choi DH, Kwon TO, Lee HS. Screening of vasorelaxant activity of some medicinal plants used in oriental medicines. J of Ethnopharmacol. 2005; 99(1):113-117. 16. Rodriguez E, Towers G, Mitchell J. Biological activities of sesquiterpene lactones. Phytochemistry. 1976; 15(11):1573–1580.
  • 17. Harada A, Sakata K, Ina H, Ina K. Isolation and identification of xanthatin as an anti-attaching repellent against blue mussel. Agric Biol Chem. 1985; 49(6):1887–1888. 18. Kawazu K, Nakajima S, Ariwa M. Xanthumin and 8-epi-xanthatin as insect development inhibitors from Xanthium canadense Mill. Experientia. 1979; 35(10):1294–1295. 19. Kim H, Lee I, Yeo S, Seong L, Yu T. Isolation and characterization of antitumor agents from Xanthium strumarium L. Korean J Biotechnol Bioeng. 2003; 18:324-328.
  • 20. Lavault M, Landreau A, Larcher G, Bouchara JP, Pagniez F, Le P, et al. Antileishmanial and antifungal activities of xanthanolides isolated from Xanthium macrocarpum. Fitoterapia. 2005; 76(3):363-366.
  • 21. Roussakis C, Chinou I, Vayas C, Harvala C, Verbist J. Cytotoxic activity of xanthatin and the crude extracts of Xanthium strumarium. Planta Med. 1994; 60(5):473.
  • 22. Sato Y, Oketani H, Yamada T, Singyouchi KI, Ohtsubo T, Kihara M, et al. A Xanthanolide with potent antibacterial activity against Methicillin-resistant Staphylococcus aureus. J Pharm Pharmacol. 1997; 49(10):1042–1044.
  • 23. Ladynina EA. Wisdom of Herbs, Herbal Cures and Homeopathy [in Russian], AIF Print, Moscow. 2003; 216-217.
  • 24. Klimakhin GI, Fonin VS, Maslyakov VY, Fadeev NB, Semikin VV, Pelgunova LA. Biochemical features of common cocklebur (Xanthıum strumarium L.) Pharmaceutical Chemistry J. 2015; 49(8):32-35.
  • 25. Zhu YP. Chinese Materia Medica: Chemistry, Pharmacology and Applications. Harwood Academic Publishers: Amsterdam; 1998.
  • 26. Kamboj A, Saluja AK. Phytopharmacological review of Xanthium strumarium L. (Cocklebur). Int J of Green Pharmacy. 2010; 4:129-139.
  • 27. Levan A, Fredga K, Sanberg AA. Nomenclature for centromeric position on chromosomes. Hereditas. 1964; 52(2):201-220.
  • 28. Romero-Zarco C. A new method for estimating karyotype asymmetry. Taxon. 1986; 35: 526-530.
  • 29. Stebbins GL. Chromosomal evolution in higher plants. London, UK: Edward Arnold (Publishers) Ltd; 1971.
  • 30. http://www.tropicos.org/Project/IPCN
  • 31. http://www.lib.kobe-u.ac.jp/infolib/meta_pub/G0000003
  • 32. Sarker AK, Datta N, Chatterjee U, Hazra D. In Love, A. (ed.):IOPB Chromosome number reports. LXXVI. Taxon. 1982; 31(3):574-598.
  • 33. Razaq ZA, Vahidy AA, Ali DH. Chromosome numbers in Compositae from Pakistan. Ann Missouri Bot Gard. 1994; 81(4):800-808.
  • 34. Bala S, Gupta G. In K. Marhold (ed.): IAPT/IOPB Chromosome data 12. Taxon. 2011; 60(6):1784-1796.
  • 35. Bala S, Gupta G. Male meiosis and chromosome number in Asteraceae family from district Kangra of H.P. (Western Himalayas). Int J Bot Res. 2013; 3(1):43-58.
  • 36. Singhal VK, Garg P, Kumar P. Cytological studies of some dicots from the hills of Mandi district (Himachal Pradesh) in Northwest Indian Himalayas. Cytologia. 2013; 78(1):1-14.
  • 37. Ishıkawa M. A list of the number of chromosomes. Bot Mag Tokyo (J Plant Res.) 1916; 30(360): 404. 38. Skalinska M, Malecka J, Izmailow R. et al. Further studies in chromosome numbers of polish angiosperms X. Acta Biol Cracov Ser Bot. 1974; 17:133-164.
  • 39. Rostoutseva TS. Chromosome numbers of some species of the family Asteraceae Dumort. Bot Zhurn. (Moscow and Leningrad) 1979; 64(4):582-589.
  • 40. Sidhu MK. Distributional and cytological studies of the weed flora of cultivable field of Patiala district (Panjab). Ph. D. Thesis, Punjabi University: Patiala -India; 1979.
  • 41. Bir SS, Sidhu M. Cyto-palynological studies on weed flora of cultivable lands of Patiala district (Punjab). J Palynology. 1980; 16:85-105.
  • 42. Love A, Love D. In Love, A.(ed.): IOPB Chromosome number reports. LXXV. Taxon. 1982; 31(2): 342-368.
  • 43. Lomonosova MN. In K. Marhold and I. Breitwieser (eds.): IAPT/IOPB Chromosome data 16. Taxon. 2013; 62(6):1357.
  • 44. Bakale VL, Srinivasu T. Mitotic and karyological studies in Xanthium strumarium L. Adv Plant Sci. 1988; 1:201-207.
  • 45. Mathew A, Mathew PH. Cytological studies on the south Indian Compositae. Glimpses Plant Res. 1998; 8:1-177.
  • 46.Probatova NS, Sokolovskaia AP. Chromosome numbers in some representatives of the families Asclepiadaceae, Asteraceae, Boraginaceae, Chenopodiaceae, Lamiaceae, Oleaceae, Onagraceae, Scrophularoaceae, Solanaceae, Urticaceae from the Soviet Far East. (in Russian). Bot. Zhurn. (Moscow and Leningrad). 1990; 75(11):1619-1622.
  • 47. Baltisbarger M. Chromosome numbers in some species from Greece. Bot Chron (Patras). 1994; 11:15-29.
  • 48. Jose JC, Mathew PM. Chromosome numbers in the south Indian Heliantheae (Compositae). Compositae Newslett. 1995; 27:7-10. 49. Badr A, Kamel EA, Garcia-Jacas N. Chromosome stusies in the Egyptian Flora. IV. Karyotype features of some species in subfamily Asteroideae (Asteraceae). Compositae Newslett. 1997; 30:15-28.
  • 50. Bennett MD, Leitch IJ, Hanson L. DNA amounts in two samples of Angiosperm weeds. Ann Bot. 1998; 82 (Suppl. A):121-134.
  • 51. Joshi KK, Joshi SD. Genetic heritage of medicinal and aromatic plants of Nepal Himalayas, Buddha Academic Publishers and Distributers Pvt. Ltd, Kathmandu: Nepal; 2001.
  • 52. Alam SS, Sukur, MB, Zaman, MY. Karyotype analysis in 2 morphological forms of Xanthium strumarium L. Cytologia. 2011; 76(4):483-488.
  • 53. Gupta RC, Kataria V, Mehra A. Cytomorphological studies in some gamopetalous species of Western Himalaya: An attempt to add new or varied cytotypes. Chromosome Botany. 2012; 7(2):59-65.
  • 54. Chepinoga VV, Gnutikov AA, Lubogoschinsky PI, Isaikina MM, Konovalov AS. In Marhold, K.(ed.):IAPT/IOPB Chromosome data 13. Taxon. 2012; 61(4):891.
Konular Fen
Dergi Bölümü Original Articles

Yazar: Gulden Dogan
Ülke: Turkey

Yazar: Yasar Kiran
Kurum: Firat University, Faculty of Science, Dept. of Biology, Elazig
Ülke: Turkey

Bibtex @araştırma makalesi { nsd332746, journal = {Natural Science and Discovery}, issn = {2149-6307}, eissn = {2149-6307}, address = {Lycia Press Inc.}, year = {2017}, volume = {3}, pages = {39 - 43}, doi = {10.20863/nsd.332746}, title = {Karyotype analysis of Common Cocklebur (Xanthium strumarium L.)}, key = {cite}, author = {Kiran, Yasar and Dogan, Gulden} }
APA Dogan, G , Kiran, Y . (2017). Karyotype analysis of Common Cocklebur (Xanthium strumarium L.). Natural Science and Discovery, 3 (3), 39-43. DOI: 10.20863/nsd.332746
MLA Dogan, G , Kiran, Y . "Karyotype analysis of Common Cocklebur (Xanthium strumarium L.)". Natural Science and Discovery 3 (2017): 39-43 <http://dergipark.gov.tr/nsd/issue/30487/332746>
Chicago Dogan, G , Kiran, Y . "Karyotype analysis of Common Cocklebur (Xanthium strumarium L.)". Natural Science and Discovery 3 (2017): 39-43
RIS TY - JOUR T1 - Karyotype analysis of Common Cocklebur (Xanthium strumarium L.) AU - Gulden Dogan , Yasar Kiran Y1 - 2017 PY - 2017 N1 - doi: 10.20863/nsd.332746 DO - 10.20863/nsd.332746 T2 - Natural Science and Discovery JF - Journal JO - JOR SP - 39 EP - 43 VL - 3 IS - 3 SN - 2149-6307-2149-6307 M3 - doi: 10.20863/nsd.332746 UR - http://dx.doi.org/10.20863/nsd.332746 Y2 - 2017 ER -
EndNote %0 Natural Science and Discovery Karyotype analysis of Common Cocklebur (Xanthium strumarium L.) %A Gulden Dogan , Yasar Kiran %T Karyotype analysis of Common Cocklebur (Xanthium strumarium L.) %D 2017 %J Natural Science and Discovery %P 2149-6307-2149-6307 %V 3 %N 3 %R doi: 10.20863/nsd.332746 %U 10.20863/nsd.332746
ISNAD Dogan, Gulden , Kiran, Yasar . "Karyotype analysis of Common Cocklebur (Xanthium strumarium L.)". Natural Science and Discovery 3 / 3 (Eylül 2017): 39-43. http://dx.doi.org/10.20863/nsd.332746