Yıl 2018, Cilt 33, Sayı 2, Sayfalar 105 - 115 2018-06-22

Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas
Fasulye üretim alanlarında hastalığa neden olan Xanthomonas axonopodis pv. phaseoli ve Pseudomonas savastonoi pv. phaseolicola’nın izolasyonu ve tanılanması

Murat Ozturk [1] , Hasan Murat AKSOY [2]

71 138

In 2013-2014, surveys were conducted in order to investigate the presence of Xanthomonas axonopodis pv. phaseoli (Xap) (common blight) and Pseudomonas savastonoi pv. phaseolicola (Psp) (halo blight) in common bean producing areas of Samsun province. Eight Xap and 5 Psp isolates were identified from single isolated pure colonies of the bacteria by means of biochemical, pathogenicity, molecular and 16S rDNA sequencing studies. Phylogenetic tree analysis demonstrated that the isolates of Xap and Psp obtained were clustered with their type strains. Göynük 98 was the most susceptible cultivar to Xap and Psp isolates among the five common bean cultivars tested in pathogenicity assays and selected for virulence assays. This work proved the presence of both disease agents in Samsun province, which supplies about 20 % of the total fresh bean production in Turkey, with molecular studies for the first time.

2013-2014 yıllarında, Samsun ili fasulye üretim alanlarında adi yanıklığa neden olan Xanthomonas axonopodis pv. phaseoli (Xap) ve hale yanıklığına neden olan Pseudomonas savastonoi pv. phaseolicola (Psp)’nin belirlenmesi için surveyler yapılmıştır. Elde edilen tek ve saf kolonilerle yapılan biyokimyasal, patojenisite, moleküler ve 16S rDNA sekanslama çalışmaları sonucunda 8 Xap ve 5 Psp izolatı tanılanmıştır. Xap ve Psp izolatları filogenetik ağaç analizinde tip kültürleri ile birlikte yer almıştır. Göynük 98 fasulye çeşidi in vitro patojenisite denemelerinde en hassas çeşit olarak belirlenmiştir ve virülens denemesi için kullanılmıştır. Yapılan bu çalışmada, Türkiye taze fasulye üretiminin % 20’sinin gerçekleştiği Samsun ilinde her iki hastalık etmenin varlığı moleküler çalışmalarla ilk kez ortaya konmuştur.

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Birincil Dil en
Konular Yaşam Bilimleri
Dergi Bölümü Bitki Koruma
Yazarlar

Yazar: Murat Ozturk (Sorumlu Yazar)
Kurum: Department of Plant Protection, Faculty of Agriculture, Ondokuz Mayis University, Samsun, Turkey
Ülke: Turkey


Yazar: Hasan Murat AKSOY
Kurum: Department of Plant Protection, Faculty of Agriculture, Ondokuz Mayis University, Samsun, Turkey
Ülke: Turkey


Bibtex @araştırma makalesi { omuanajas393903, journal = {Anadolu Tarım Bilimleri Dergisi}, issn = {1308-8750}, eissn = {1308-8769}, address = {Ondokuz Mayıs Üniversitesi}, year = {2018}, volume = {33}, pages = {105 - 115}, doi = {10.7161/omuanajas.393903}, title = {Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas}, key = {cite}, author = {Ozturk, Murat and AKSOY, Hasan Murat} }
APA Ozturk, M , AKSOY, H . (2018). Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas. Anadolu Tarım Bilimleri Dergisi, 33 (2), 105-115. DOI: 10.7161/omuanajas.393903
MLA Ozturk, M , AKSOY, H . "Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas". Anadolu Tarım Bilimleri Dergisi 33 (2018): 105-115 <http://dergipark.gov.tr/omuanajas/issue/37594/393903>
Chicago Ozturk, M , AKSOY, H . "Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas". Anadolu Tarım Bilimleri Dergisi 33 (2018): 105-115
RIS TY - JOUR T1 - Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas AU - Murat Ozturk , Hasan Murat AKSOY Y1 - 2018 PY - 2018 N1 - doi: 10.7161/omuanajas.393903 DO - 10.7161/omuanajas.393903 T2 - Anadolu Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 105 EP - 115 VL - 33 IS - 2 SN - 1308-8750-1308-8769 M3 - doi: 10.7161/omuanajas.393903 UR - http://dx.doi.org/10.7161/omuanajas.393903 Y2 - 2018 ER -
EndNote %0 Anadolu Tarım Bilimleri Dergisi Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas %A Murat Ozturk , Hasan Murat AKSOY %T Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas %D 2018 %J Anadolu Tarım Bilimleri Dergisi %P 1308-8750-1308-8769 %V 33 %N 2 %R doi: 10.7161/omuanajas.393903 %U 10.7161/omuanajas.393903
ISNAD Ozturk, Murat , AKSOY, Hasan Murat . "Fasulye üretim alanlarında hastalığa neden olan Xanthomonas axonopodis pv. phaseoli ve Pseudomonas savastonoi pv. phaseolicola’nın izolasyonu ve tanılanması". Anadolu Tarım Bilimleri Dergisi 33 / 2 (Haziran 2018): 105-115. http://dx.doi.org/10.7161/omuanajas.393903