Yıl 2018, Cilt 22, Sayı 1, Sayfalar 7 - 12 2017-12-26

Biochemical Characterization of Lipases Obtained from Acinetobacter psychrotolerans Strains

Hatice KATI [1] , Şule SEREN [2]

702 157

In this study, extracellular lipases obtained from Acinetobacter psychrotolerans strains (Xg1 and Xg2) were characterized. The effects of varying pH values (3.0-10.0) and various temperatures (10-90 °C) on lipase activities were examined. Also the effects of different metal ions, organic solvents and detergents on lipases were studied. The extracellular crude lipases were concentrated using ultrafiltration. Zymogram analysis of these lipases was performed. Lipases exhibited maximum activity at pH 8 and 30 °C.  While lipase obtained from the Xg1 strain exhibited the highest stability in the presence of various organic solvents, including hexane, ethyl acetate, chloroform and N,N dietil formamide, lipase obtained from the Xg2 strain was sensitive in the presence of isopropanol, acetonitrile, and butan-1-ol. The lipases of the Xg1 and Xg2 strains were inhibited in the presence of Cu2+ and Zn2+. Also, the lipase of the Xg1 strain was inhibited in the presence of Fe3+. In the presence of EDTA, the lipase activities of the Xg1 and Xg2 strains were partially inhibited. In presence of SDS, they were exactly inhibited. According to the zymogram results, the molecular weights of the lipases obtained from the Acinetobacter psychrotolerans Xg1 and Xg2 strains have been found approximately 37 and 30 kDa, respectively.
Acinetobacter, Lipase; Charecterization
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Dergi Bölümü Makaleler
Yazarlar

Yazar: Hatice KATI

Yazar: Şule SEREN

Bibtex @ { sdufenbed425660, journal = {Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi}, issn = {}, eissn = {1308-6529}, address = {Süleyman Demirel Üniversitesi}, year = {2017}, volume = {22}, pages = {7 - 12}, doi = {10.19113/sdufbed.10020}, title = {Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains}, key = {cite}, author = {SEREN, Şule and KATI, Hatice} }
APA KATI, H , SEREN, Ş . (2017). Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22 (1), 7-12. Retrieved from http://dergipark.gov.tr/sdufenbed/issue/37055/425660
MLA KATI, H , SEREN, Ş . "Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains". Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 (2017): 7-12 <http://dergipark.gov.tr/sdufenbed/issue/37055/425660>
Chicago KATI, H , SEREN, Ş . "Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains". Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 (2017): 7-12
RIS TY - JOUR T1 - Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains AU - Hatice KATI , Şule SEREN Y1 - 2017 PY - 2017 N1 - DO - T2 - Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi JF - Journal JO - JOR SP - 7 EP - 12 VL - 22 IS - 1 SN - -1308-6529 M3 - UR - Y2 - 2018 ER -
EndNote %0 Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains %A Hatice KATI , Şule SEREN %T Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains %D 2017 %J Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi %P -1308-6529 %V 22 %N 1 %R %U
ISNAD KATI, Hatice , SEREN, Şule . "Biochemical Characterization of Lipases Obtained from <i>Acinetobacter psychrotolerans</i> Strains". Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 / 1 (Aralık 2017): 7-12.