Yıl 2018, Cilt 25, Sayı 2, Sayfalar 157 - 166 2018-05-01

Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi
In vitro cytotoxic evaluation of conventional denture base material and soft lining material using colorimetric MTT assay

Canan Akay [1] , Merve Çakırbay Tanış [2]

46 97

Özet

Amaç: Bu çalışmada protez yapımında kullanılan geleneksel kaide materyali ile yumuşak astar materyalinin fare fibroblast hücreleri üzerinde zamanla meydana gelen sitotoksik etkilerinin değerlendirilmesi amaçlandı.

Materyal ve Metod: Protez kaide materyali (rodeks) ve yumuşak astar materyalinin (dentusil) disk şekilli test numuneleri üreticinin talimatlarına göre aseptik şartlar altında hazırlandı.. Örnekler, ağız ortamını taklit etmek için 5.000 termal döngüye tabi tutuldu. Yaşlanma prosedürlerini takiben, materyallerin sitotoksik etkisi, 24 saat, 48 saat ve 72 saatlik hücre inkubasyon döneminden sonra L929 fare fibroblast hücreleri kullanılarak [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] testi ile değerlendirildi. Her grup için hücre canlılığı değerleri hesaplandı. Verilerin istatistiksel analizi, iki yönlü tekrarlanan bir ölçüm yöntemi kullanılarak gerçekleştirildi. (P <0.001)

Bulgular: 24 saat ve 48 saat inkubasyon periyodunda yumuşak astar materyali, 72 saat inkübasyon periyodunda ise kaide materyali daha fazla hücre canlılığı göstermiştir. İstatistiksel olarak iki materyal arasında anlamlı fark bulunmuştur. İnkübasyon periyotları arasında ise 24 saat inkübe edilen grup 48 saat ve 72 saatden istatistiksel olarak farklıdır. 72 saat ve 48 saat arasında anlamlı fark bulunamamıştır.

Sonuç: Kaide materyallerinin altına kullanmış olduğumuz yumuşak astar materyali kaide materyaline göre daha biyouyumludur.


Abstract

Purpose: In this study, it was aimed to evaluate the time course of cytotoxic effects of the conventional base material and soft lining material on the mouse fibroblast cells.

Material and Method:

Disc-shaped test samples of denture base material (rodex) and soft lining material( dentusil) were fabricated according to manufacturers' instructions under aseptic conditions. The samples were subjected to 5,000 thermal cycling to mimic the oral environment. Following aging procedures, the cytotoxic effect of the materials was assessed by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide]  assay using L929 mouse fibroblast cells after 24 h, 48 h and 72 h cell incubation period. Cell viability values were calculated for each group. Statistical analysis of the data was performed using a two-way repeated measurement method. (p<0,001)

Results: For 24 hours and 48 hours incubation period soft lining material, and  for 72 hour incubation period the base material showed more cell viability. Statistically, there was a significant difference between the two materials. During the incubation period, the group incubated 24 hours is statistically different from 48 hours and 72 hours. No significant difference was found between 72 hours and 48 hours.

Conclusion: The soft lining material we used under the base materials is more biocompatible than the base material.


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Konular Sağlık Bilimleri, Tıp Bilimleri
Dergi Bölümü Araştırma Makaleleri
Yazarlar

Orcid: https://orcid.org/0000-0003-2781-8710
Yazar: Canan Akay
Kurum: ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, PROTETİK DİŞ TEDAVİSİ ANABİLİM DALI
Ülke: Turkey


Orcid: https://orcid.org/0000-0001-5698-8220
Yazar: Merve Çakırbay Tanış
Kurum: Ministry of Health, Karapürçek Oral and Dental Health Clinics, Ankara, Turkey
Ülke: Turkey


Bibtex @araştırma makalesi { sdutfd337797, journal = {SDÜ Tıp Fakültesi Dergisi}, issn = {1300-7416}, eissn = {2602-2109}, address = {Süleyman Demirel Üniversitesi}, year = {2018}, volume = {25}, pages = {157 - 166}, doi = {10.17343/sdutfd.337797}, title = {Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi}, key = {cite}, author = {Çakırbay Tanış, Merve and Akay, Canan} }
APA Akay, C , Çakırbay Tanış, M . (2018). Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi. SDÜ Tıp Fakültesi Dergisi, 25 (2), 157-166. DOI: 10.17343/sdutfd.337797
MLA Akay, C , Çakırbay Tanış, M . "Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi". SDÜ Tıp Fakültesi Dergisi 25 (2018): 157-166 <http://dergipark.gov.tr/sdutfd/issue/37318/337797>
Chicago Akay, C , Çakırbay Tanış, M . "Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi". SDÜ Tıp Fakültesi Dergisi 25 (2018): 157-166
RIS TY - JOUR T1 - Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi AU - Canan Akay , Merve Çakırbay Tanış Y1 - 2018 PY - 2018 N1 - doi: 10.17343/sdutfd.337797 DO - 10.17343/sdutfd.337797 T2 - SDÜ Tıp Fakültesi Dergisi JF - Journal JO - JOR SP - 157 EP - 166 VL - 25 IS - 2 SN - 1300-7416-2602-2109 M3 - doi: 10.17343/sdutfd.337797 UR - http://dx.doi.org/10.17343/sdutfd.337797 Y2 - 2017 ER -
EndNote %0 SDÜ Tıp Fakültesi Dergisi Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi %A Canan Akay , Merve Çakırbay Tanış %T Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi %D 2018 %J SDÜ Tıp Fakültesi Dergisi %P 1300-7416-2602-2109 %V 25 %N 2 %R doi: 10.17343/sdutfd.337797 %U 10.17343/sdutfd.337797
ISNAD Akay, Canan , Çakırbay Tanış, Merve . "Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi". SDÜ Tıp Fakültesi Dergisi 25 / 2 (Mayıs 2018): 157-166. http://dx.doi.org/10.17343/sdutfd.337797