Yıl 2013, Cilt 37, Sayı 5, Sayfalar 507 - 513 2014-05-16

Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy
Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy

Mahalingam ANBARASU [1] , Ramangalingam KIRUBAGARAN [2] , Gopal DHARANI [3] , Thanumalaya SUBRAMONIAM [4]

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The architecture of testicular lobules, morphology of spermatozoa, and spermatophore in the mud crab, Scylla serrata, are described for the first time employing atomic force microscopy (AFM). A fast and simple squash preparation protocol was followed using crustacean physiological saline without distorting the natural structure. Observations provided new insight into the arrangement of lobules and morphology of testicular assembly. It consists of a close network of lobules of different sizes in a radial manner on radially branching basal tubules. Budding of new lobules is another distinguishable feature noted in the network of testicular organization. A thin membranous envelope of the lobule with undulating processes offered indirect evidence on the possible storage of sperm cells within the lumen of the lobule. Spermatozoa of S. serrata were of different shapes and sizes, and, depending on the maturational status, they displayed changes in the acrosome vesicle with a significant amount of cytoplasm. Vertical constrictions found in the testicular lobules suggest propulsive extrusion of spermatophoric masses into the central lumen. These observations necessitate further study using AFM and involving molecular probes to elucidate the polymorphic nature of male germinal cells, mechanisms of spermatophore formation, and the phylogenetic relationship among crustaceans in general.
The architecture of testicular lobules, morphology of spermatozoa, and spermatophore in the mud crab, Scylla serrata, are described for the first time employing atomic force microscopy (AFM). A fast and simple squash preparation protocol was followed using crustacean physiological saline without distorting the natural structure. Observations provided new insight into the arrangement of lobules and morphology of testicular assembly. It consists of a close network of lobules of different sizes in a radial manner on radially branching basal tubules. Budding of new lobules is another distinguishable feature noted in the network of testicular organization. A thin membranous envelope of the lobule with undulating processes offered indirect evidence on the possible storage of sperm cells within the lumen of the lobule. Spermatozoa of S. serrata were of different shapes and sizes, and, depending on the maturational status, they displayed changes in the acrosome vesicle with a significant amount of cytoplasm. Vertical constrictions found in the testicular lobules suggest propulsive extrusion of spermatophoric masses into the central lumen. These observations necessitate further study using AFM and involving molecular probes to elucidate the polymorphic nature of male germinal cells, mechanisms of spermatophore formation, and the phylogenetic relationship among crustaceans in general.
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Yazar: Mahalingam ANBARASU
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Yazar: Ramangalingam KIRUBAGARAN
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Yazar: Gopal DHARANI
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Yazar: Thanumalaya SUBRAMONIAM
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Bibtex @ { tbtkbiology139554, journal = {Turkish Journal of Biology}, issn = {1300-0152}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {507 - 513}, doi = {10.3906/biy-1203-59}, title = {Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy}, key = {cite}, author = {SUBRAMONIAM, Thanumalaya and KIRUBAGARAN, Ramangalingam and ANBARASU, Mahalingam and DHARANI, Gopal} }
APA ANBARASU, M , KIRUBAGARAN, R , DHARANI, G , SUBRAMONIAM, T . (2014). Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy. Turkish Journal of Biology, 37 (5), 507-513. DOI: 10.3906/biy-1203-59
MLA ANBARASU, M , KIRUBAGARAN, R , DHARANI, G , SUBRAMONIAM, T . "Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy". Turkish Journal of Biology 37 (2014): 507-513 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139554>
Chicago ANBARASU, M , KIRUBAGARAN, R , DHARANI, G , SUBRAMONIAM, T . "Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy". Turkish Journal of Biology 37 (2014): 507-513
RIS TY - JOUR T1 - Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy AU - Mahalingam ANBARASU , Ramangalingam KIRUBAGARAN , Gopal DHARANI , Thanumalaya SUBRAMONIAM Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1203-59 DO - 10.3906/biy-1203-59 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 507 EP - 513 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1203-59 UR - http://dx.doi.org/10.3906/biy-1203-59 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy %A Mahalingam ANBARASU , Ramangalingam KIRUBAGARAN , Gopal DHARANI , Thanumalaya SUBRAMONIAM %T Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1203-59 %U 10.3906/biy-1203-59