Cilt 37, Sayı 5, Sayfalar 514 - 519 2014-05-16

Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın
Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın

Erman ORYAŞIN [1] , Hacı Halil BIYIK [2] , Gamze BAŞBÜLBÜL [3] , Bülent BOZDOĞAN [4]

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The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. The results were compared with the susceptibility patterns of hospital enterococci reported in a previous study. Samples from 9 environmental sources were cultured, and species were identified using 16S rRNA sequencing. Antimicrobial susceptibilities were determined by the agar dilution method. The minimum inhibitory concentrations were tested for vancomycin, tetracycline, teicoplanin, ampicillin, gentamicin, erythromycin, rifampicin, clindamycin, chloramphenicol, and ciprofloxacin. Resistance genes and transposons were determined by polymerase chain reaction. The transferability of resistance was tested by conjugation. A total of 57 enterococci were isolated. All erythromycin-resistant enterococci had ermB and all tetracycline-resistant isolates had tetM genes. Macrolide and tetracycline resistances were transferable by conjugation to Enterococcus faecalis JH2-2. Transconjugants were confirmed by pulsed-field gel electrophoresis analysis. Our study showed the existence of antimicrobial resistance among bacteria isolated from environmental samples. All isolates were resistant to at least one antibiotic tested. Transferability of resistance genes in environmental enterococci showed the importance of environmental resistance for public health and the potential of resistance gene dissemination. Although the sample collection times of environmental and hospital isolates were different, the possibility exists that the difference in susceptibility patterns may be the result of differences in antimicrobial pressure in hospitals and in the environment, and this should be studied further.
The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci. The results were compared with the susceptibility patterns of hospital enterococci reported in a previous study. Samples from 9 environmental sources were cultured, and species were identified using 16S rRNA sequencing. Antimicrobial susceptibilities were determined by the agar dilution method. The minimum inhibitory concentrations were tested for vancomycin, tetracycline, teicoplanin, ampicillin, gentamicin, erythromycin, rifampicin, clindamycin, chloramphenicol, and ciprofloxacin. Resistance genes and transposons were determined by polymerase chain reaction. The transferability of resistance was tested by conjugation. A total of 57 enterococci were isolated. All erythromycin-resistant enterococci had ermB and all tetracycline-resistant isolates had tetM genes. Macrolide and tetracycline resistances were transferable by conjugation to Enterococcus faecalis JH2-2. Transconjugants were confirmed by pulsed-field gel electrophoresis analysis. Our study showed the existence of antimicrobial resistance among bacteria isolated from environmental samples. All isolates were resistant to at least one antibiotic tested. Transferability of resistance genes in environmental enterococci showed the importance of environmental resistance for public health and the potential of resistance gene dissemination. Although the sample collection times of environmental and hospital isolates were different, the possibility exists that the difference in susceptibility patterns may be the result of differences in antimicrobial pressure in hospitals and in the environment, and this should be studied further.
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Yazar: Erman ORYAŞIN
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Yazar: Hacı Halil BIYIK
E-posta:

Yazar: Gamze BAŞBÜLBÜL
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Yazar: Bülent BOZDOĞAN
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Bibtex @ { tbtkbiology139555, journal = {Turkish Journal of Biology}, issn = {1300-0152}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {514 - 519}, doi = {10.3906/biy-1203-3}, title = {Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın}, language = {en}, key = {cite}, author = {BIYIK, Hacı Halil and BAŞBÜLBÜL, Gamze and ORYAŞIN, Erman and BOZDOĞAN, Bülent} } @ { tbtkbiology139555, journal = {Turkish Journal of Biology}, issn = {1300-0152}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {514 - 519}, doi = {10.3906/biy-1203-3}, title = {Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın}, language = {tr}, key = {cite}, author = {BIYIK, Hacı Halil and BAŞBÜLBÜL, Gamze and ORYAŞIN, Erman and BOZDOĞAN, Bülent} }
APA ORYAŞIN, E , BIYIK, H , BAŞBÜLBÜL, G , BOZDOĞAN, B . (2014). Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın. Turkish Journal of Biology, 37 (5), 514-519. DOI: 10.3906/biy-1203-3
MLA ORYAŞIN, E , BIYIK, H , BAŞBÜLBÜL, G , BOZDOĞAN, B . "Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın". Turkish Journal of Biology 37 (2014): 514-519 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139555>
Chicago ORYAŞIN, E , BIYIK, H , BAŞBÜLBÜL, G , BOZDOĞAN, B . "Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın". Turkish Journal of Biology 37 (2014): 514-519
RIS TY - JOUR T1 - Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın AU - Erman ORYAŞIN , Hacı Halil BIYIK , Gamze BAŞBÜLBÜL , Bülent BOZDOĞAN Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1203-3 DO - 10.3906/biy-1203-3 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 514 EP - 519 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1203-3 UR - http://dx.doi.org/10.3906/biy-1203-3 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın %A Erman ORYAŞIN , Hacı Halil BIYIK , Gamze BAŞBÜLBÜL , Bülent BOZDOĞAN %T Antimicrobial susceptibility patterns of environmental and hospital isolations of enterococci in Aydın %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1203-3 %U 10.3906/biy-1203-3