Cilt 37, Sayı 5, Sayfalar 520 - 529 2014-05-16

Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes
Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes

Magda ALY [1] , Sanaa TORK [2] , Saleh AL-GARNI [3] , Reda ALLAM [4]

211 709

Uricase plays an important role in nitrogen metabolism and can be used medically as a diagnostic reagent. From soil, wastewater, and poultry waste samples collected in Jeddah, 49 bacterial isolates were obtained on either nutrient agar or starch nitrate agar. All the obtained isolates were screened on minimal medium containing 0.5% uric acid for uricase production. The most active bacterium (isolate UR10) produced about 0.5 U/mL of intracellular uricase and was identified as a species belonging to the genus Streptomyces using morphological, physiological, and biochemical characters. By 16S rDNA, it was identified as Streptomyces exfoliatus UR10. Maximum uricase production was obtained using medium 2 with 0.2% uric acid as an inducer, an initial pH of 6.5, and an incubation temperature of 37 °C at 100 rpm. At the end of the incubation period, the cells were collected and disturbed, and the uricase enzyme was precipitated by ammonium sulfate. The enzyme was purified using different column chromatography methods, and the molecular weight of the purified uricase was determined by SDS-PAGE electrophoresis. The optimum temperature for maximum uricase activity was 45 °C; the optimum pH was 8. Co2+, Ni2+, Zn2+, Cu2+, and Pb2+ decreased the enzyme activity, whereas Ca2+, Mn2+, Mg2+, and Fe2+ stimulated it. In conclusion, uricase was produced by Streptomyces in a medium containing uric acid as inducer, and this enzyme can be used to detect and quantify uric acid in urine and/or blood.
Uricase plays an important role in nitrogen metabolism and can be used medically as a diagnostic reagent. From soil, wastewater, and poultry waste samples collected in Jeddah, 49 bacterial isolates were obtained on either nutrient agar or starch nitrate agar. All the obtained isolates were screened on minimal medium containing 0.5% uric acid for uricase production. The most active bacterium (isolate UR10) produced about 0.5 U/mL of intracellular uricase and was identified as a species belonging to the genus Streptomyces using morphological, physiological, and biochemical characters. By 16S rDNA, it was identified as Streptomyces exfoliatus UR10. Maximum uricase production was obtained using medium 2 with 0.2% uric acid as an inducer, an initial pH of 6.5, and an incubation temperature of 37 °C at 100 rpm. At the end of the incubation period, the cells were collected and disturbed, and the uricase enzyme was precipitated by ammonium sulfate. The enzyme was purified using different column chromatography methods, and the molecular weight of the purified uricase was determined by SDS-PAGE electrophoresis. The optimum temperature for maximum uricase activity was 45 °C; the optimum pH was 8. Co2+, Ni2+, Zn2+, Cu2+, and Pb2+ decreased the enzyme activity, whereas Ca2+, Mn2+, Mg2+, and Fe2+ stimulated it. In conclusion, uricase was produced by Streptomyces in a medium containing uric acid as inducer, and this enzyme can be used to detect and quantify uric acid in urine and/or blood.
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Yazar: Magda ALY
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Yazar: Sanaa TORK
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Yazar: Saleh AL-GARNI
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Yazar: Reda ALLAM
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Bibtex @ { tbtkbiology139556, journal = {Turkish Journal of Biology}, issn = {1300-0152}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {520 - 529}, doi = {10.3906/biy-1206-3}, title = {Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes}, language = {en}, key = {cite}, author = {TORK, Sanaa and AL-GARNI, Saleh and ALLAM, Reda and ALY, Magda} } @ { tbtkbiology139556, journal = {Turkish Journal of Biology}, issn = {1300-0152}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {520 - 529}, doi = {10.3906/biy-1206-3}, title = {Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes}, language = {tr}, key = {cite}, author = {TORK, Sanaa and AL-GARNI, Saleh and ALLAM, Reda and ALY, Magda} }
APA ALY, M , TORK, S , AL-GARNI, S , ALLAM, R . (2014). Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes. Turkish Journal of Biology, 37 (5), 520-529. DOI: 10.3906/biy-1206-3
MLA ALY, M , TORK, S , AL-GARNI, S , ALLAM, R . "Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes". Turkish Journal of Biology 37 (2014): 520-529 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139556>
Chicago ALY, M , TORK, S , AL-GARNI, S , ALLAM, R . "Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes". Turkish Journal of Biology 37 (2014): 520-529
RIS TY - JOUR T1 - Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes AU - Magda ALY , Sanaa TORK , Saleh AL-GARNI , Reda ALLAM Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1206-3 DO - 10.3906/biy-1206-3 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 520 EP - 529 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1206-3 UR - http://dx.doi.org/10.3906/biy-1206-3 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes %A Magda ALY , Sanaa TORK , Saleh AL-GARNI , Reda ALLAM %T Production and characterization of uricase from Streptomyces exfoliatus UR10 isolated from farm wastes %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1206-3 %U 10.3906/biy-1206-3