Yıl 2013, Cilt 37, Sayı 5, Sayfalar 530 - 537 2014-05-16

Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea
Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea

Kutsal KESİCİ [1] , İnci TÜNEY [2] , Doğuş ZEREN [3] , Mustafa GÜDEN [4] , Atakan SUKATAR [5]

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Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then we amplified ITS regions using a conventional polymerase chain reaction (PCR), sequenced the PCR products, and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia delicatissima, Achnanthes taeniata, Amphora coffeaeformis, and Cylindrotheca closterium. We think that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.
Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then we amplified ITS regions using a conventional polymerase chain reaction (PCR), sequenced the PCR products, and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia delicatissima, Achnanthes taeniata, Amphora coffeaeformis, and Cylindrotheca closterium. We think that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.
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Yazar: Kutsal KESİCİ

Yazar: İnci TÜNEY

Yazar: Doğuş ZEREN

Yazar: Mustafa GÜDEN

Yazar: Atakan SUKATAR

Bibtex @ { tbtkbiology139557, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {530 - 537}, doi = {10.3906/biy-1205-40}, title = {Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea}, key = {cite}, author = {TÜNEY, İnci and GÜDEN, Mustafa and KESİCİ, Kutsal and ZEREN, Doğuş and SUKATAR, Atakan} }
APA KESİCİ, K , TÜNEY, İ , ZEREN, D , GÜDEN, M , SUKATAR, A . (2014). Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea. Turkish Journal of Biology, 37 (5), 530-537. DOI: 10.3906/biy-1205-40
MLA KESİCİ, K , TÜNEY, İ , ZEREN, D , GÜDEN, M , SUKATAR, A . "Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea". Turkish Journal of Biology 37 (2014): 530-537 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139557>
Chicago KESİCİ, K , TÜNEY, İ , ZEREN, D , GÜDEN, M , SUKATAR, A . "Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea". Turkish Journal of Biology 37 (2014): 530-537
RIS TY - JOUR T1 - Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea AU - Kutsal KESİCİ , İnci TÜNEY , Doğuş ZEREN , Mustafa GÜDEN , Atakan SUKATAR Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1205-40 DO - 10.3906/biy-1205-40 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 530 EP - 537 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1205-40 UR - http://dx.doi.org/10.3906/biy-1205-40 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea %A Kutsal KESİCİ , İnci TÜNEY , Doğuş ZEREN , Mustafa GÜDEN , Atakan SUKATAR %T Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1205-40 %U 10.3906/biy-1205-40
ISNAD KESİCİ, Kutsal , TÜNEY, İnci , ZEREN, Doğuş , GÜDEN, Mustafa , SUKATAR, Atakan . "Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea". Turkish Journal of Biology 37 / 5 (Mayıs 2014): 530-537. http://dx.doi.org/10.3906/biy-1205-40