Yıl 2013, Cilt 37, Sayı 5, Sayfalar 556 - 564 2014-05-16

Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains
Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains

Dorota WOJNICZ [1] , Dorota TICHACZEK-GOSKA [2] , Marta KICIA [3]

173 334

This work was aimed at determining the possible antimicrobial potential of asiatic acid (AA) and ursolic acid (UA) against clinical uropathogenic Escherichia coli strains (UPECs). Phylogenetic affiliations of UPECs and virulence-associated gene carriage have been identified. PCR analysis revealed that the studied E. coli strains belong to phylogenetic group B2. All bacterial isolates were grouped into 4 pathotypes. The following were determined: the minimum inhibitory concentrations (MICs) according to CLSI; P-fimbriae synthesis, based on erythrocyte agglutination; curli fibers, by using YESCA agar with congo red; swimming motility, in motility agar; and alpha-hemolysin, on sheep's-blood agar plates. The differences in antigrowth activities of AA and UA were recorded. Inhibition of P-fimbriae, curli fibers, and alpha-hemolysin production after exposure of UPECs to both AA and UA were observed. Reduction of bacterial motility was also noticed. Our results clearly show the influence of AA and UA on virulence factors of UPEC strains. However, differences between antivirulence activities of AA and UA were not found, although a higher level of antigrowth activity of AA in comparison with UA was shown.
This work was aimed at determining the possible antimicrobial potential of asiatic acid (AA) and ursolic acid (UA) against clinical uropathogenic Escherichia coli strains (UPECs). Phylogenetic affiliations of UPECs and virulence-associated gene carriage have been identified. PCR analysis revealed that the studied E. coli strains belong to phylogenetic group B2. All bacterial isolates were grouped into 4 pathotypes. The following were determined: the minimum inhibitory concentrations (MICs) according to CLSI; P-fimbriae synthesis, based on erythrocyte agglutination; curli fibers, by using YESCA agar with congo red; swimming motility, in motility agar; and alpha-hemolysin, on sheep's-blood agar plates. The differences in antigrowth activities of AA and UA were recorded. Inhibition of P-fimbriae, curli fibers, and alpha-hemolysin production after exposure of UPECs to both AA and UA were observed. Reduction of bacterial motility was also noticed. Our results clearly show the influence of AA and UA on virulence factors of UPEC strains. However, differences between antivirulence activities of AA and UA were not found, although a higher level of antigrowth activity of AA in comparison with UA was shown.
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Yazar: Dorota WOJNICZ

Yazar: Dorota TICHACZEK-GOSKA

Yazar: Marta KICIA

Bibtex @ { tbtkbiology139560, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {556 - 564}, doi = {10.3906/biy-1208-44}, title = {Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains}, key = {cite}, author = {KICIA, Marta and WOJNICZ, Dorota and TICHACZEK-GOSKA, Dorota} }
APA WOJNICZ, D , TICHACZEK-GOSKA, D , KICIA, M . (2014). Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains. Turkish Journal of Biology, 37 (5), 556-564. DOI: 10.3906/biy-1208-44
MLA WOJNICZ, D , TICHACZEK-GOSKA, D , KICIA, M . "Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains". Turkish Journal of Biology 37 (2014): 556-564 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139560>
Chicago WOJNICZ, D , TICHACZEK-GOSKA, D , KICIA, M . "Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains". Turkish Journal of Biology 37 (2014): 556-564
RIS TY - JOUR T1 - Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains AU - Dorota WOJNICZ , Dorota TICHACZEK-GOSKA , Marta KICIA Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1208-44 DO - 10.3906/biy-1208-44 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 556 EP - 564 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1208-44 UR - http://dx.doi.org/10.3906/biy-1208-44 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains %A Dorota WOJNICZ , Dorota TICHACZEK-GOSKA , Marta KICIA %T Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1208-44 %U 10.3906/biy-1208-44
ISNAD WOJNICZ, Dorota , TICHACZEK-GOSKA, Dorota , KICIA, Marta . "Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains". Turkish Journal of Biology 37 / 5 (Mayıs 2014): 556-564. http://dx.doi.org/10.3906/biy-1208-44