Yıl 2013, Cilt 37, Sayı 5, Sayfalar 582 - 590 2014-05-16

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis
Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

Nurper GÜZ [1] , Aslı DAĞERİ [2] , Tuğba ERDOĞAN [3] , Mouzhgan MOUSAVI [4] , Şerife BAYRAM [5] , Mehmet Oktay GÜRKAN [6]

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Iron is an essential nutrient for almost all organisms, but it is also a potent toxin because it can catalyze oxidative reactions that are destructive to cells. Transferrin is an essential protein involved in iron metabolism, immunity, and vitellogenesis in insects. We have characterized a cDNA encoding a putative iron-binding transferrin (SpliTrf) in Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae), an important pest that causes extensive losses in many vegetable, fodder, and fiber crops. The structure of the putative SpliTrf showed significant homology to other insect transferrins. Gene expression of SpliTrf was examined from different adult tissues including the fat body, midgut, Malpighian tubules, nervous system, hemolymph, and ovaries, as well as from different host developmental stages. Transcripts for SpliTrf were detected in all developmental stages, but SpliTrf was found to be preferentially expressed in the fat body tissue. Furthermore, we analyzed SpliTrf expression in response to Spodoptera littoralis nucleopolyhedrovirus, SpliNPV, and Bacillus thuringiensis infection. Our results show that while SpliTrf expression is upregulated upon bacterial infection, it is downregulated upon baculoviral infection. We discuss the role of transferrin in iron metabolism as well as in host immune physiology.
Iron is an essential nutrient for almost all organisms, but it is also a potent toxin because it can catalyze oxidative reactions that are destructive to cells. Transferrin is an essential protein involved in iron metabolism, immunity, and vitellogenesis in insects. We have characterized a cDNA encoding a putative iron-binding transferrin (SpliTrf) in Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae), an important pest that causes extensive losses in many vegetable, fodder, and fiber crops. The structure of the putative SpliTrf showed significant homology to other insect transferrins. Gene expression of SpliTrf was examined from different adult tissues including the fat body, midgut, Malpighian tubules, nervous system, hemolymph, and ovaries, as well as from different host developmental stages. Transcripts for SpliTrf were detected in all developmental stages, but SpliTrf was found to be preferentially expressed in the fat body tissue. Furthermore, we analyzed SpliTrf expression in response to Spodoptera littoralis nucleopolyhedrovirus, SpliNPV, and Bacillus thuringiensis infection. Our results show that while SpliTrf expression is upregulated upon bacterial infection, it is downregulated upon baculoviral infection. We discuss the role of transferrin in iron metabolism as well as in host immune physiology.
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Yazar: Nurper GÜZ

Yazar: Aslı DAĞERİ

Yazar: Tuğba ERDOĞAN

Yazar: Mouzhgan MOUSAVI

Yazar: Şerife BAYRAM

Yazar: Mehmet Oktay GÜRKAN

Bibtex @ { tbtkbiology139563, journal = {Turkish Journal of Biology}, issn = {1300-0152}, eissn = {1303-6092}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {582 - 590}, doi = {10.3906/biy-1303-45}, title = {Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis}, key = {cite}, author = {BAYRAM, Şerife and ERDOĞAN, Tuğba and GÜZ, Nurper and MOUSAVI, Mouzhgan and GÜRKAN, Mehmet Oktay and DAĞERİ, Aslı} }
APA GÜZ, N , DAĞERİ, A , ERDOĞAN, T , MOUSAVI, M , BAYRAM, Ş , GÜRKAN, M . (2014). Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis. Turkish Journal of Biology, 37 (5), 582-590. DOI: 10.3906/biy-1303-45
MLA GÜZ, N , DAĞERİ, A , ERDOĞAN, T , MOUSAVI, M , BAYRAM, Ş , GÜRKAN, M . "Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis". Turkish Journal of Biology 37 (2014): 582-590 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139563>
Chicago GÜZ, N , DAĞERİ, A , ERDOĞAN, T , MOUSAVI, M , BAYRAM, Ş , GÜRKAN, M . "Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis". Turkish Journal of Biology 37 (2014): 582-590
RIS TY - JOUR T1 - Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis AU - Nurper GÜZ , Aslı DAĞERİ , Tuğba ERDOĞAN , Mouzhgan MOUSAVI , Şerife BAYRAM , Mehmet Oktay GÜRKAN Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1303-45 DO - 10.3906/biy-1303-45 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 582 EP - 590 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1303-45 UR - http://dx.doi.org/10.3906/biy-1303-45 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis %A Nurper GÜZ , Aslı DAĞERİ , Tuğba ERDOĞAN , Mouzhgan MOUSAVI , Şerife BAYRAM , Mehmet Oktay GÜRKAN %T Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1303-45 %U 10.3906/biy-1303-45
ISNAD GÜZ, Nurper , DAĞERİ, Aslı , ERDOĞAN, Tuğba , MOUSAVI, Mouzhgan , BAYRAM, Şerife , GÜRKAN, Mehmet Oktay . "Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis". Turkish Journal of Biology 37 / 5 (Mayıs 2014): 582-590. http://dx.doi.org/10.3906/biy-1303-45