Yıl 2013, Cilt 37, Sayı 5, Sayfalar 606 - 613 2014-05-16

Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat
Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat

Aradhika TRIPATHI [1] , Rashmi AGGARWAL [2] , Anita YADAV [3]

160 349

Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, 7 sets of primers produced remarkable induced transcripts. The highest up-regulated transcripts were observed in the resistant cultivar (HD 29), whereas the down-regulated transcripts were found in the susceptible (WL 711) cultivar. Several sets of primers were designed from the defense-related gene sequences available in the GenBank database, and of these, 4 primer sets amplified the desired specific bands. Based on the putative functions, 3 defense-related genes, namely puroindoline protein PINB (AT- KB2), \Beta-1,4-glucanase (AT- KB3), and chitinase (AT- KB7), and 1 housekeeping gene, actin (AT- KB1), were characterized.
Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, 7 sets of primers produced remarkable induced transcripts. The highest up-regulated transcripts were observed in the resistant cultivar (HD 29), whereas the down-regulated transcripts were found in the susceptible (WL 711) cultivar. Several sets of primers were designed from the defense-related gene sequences available in the GenBank database, and of these, 4 primer sets amplified the desired specific bands. Based on the putative functions, 3 defense-related genes, namely puroindoline protein PINB (AT- KB2), \Beta-1,4-glucanase (AT- KB3), and chitinase (AT- KB7), and 1 housekeeping gene, actin (AT- KB1), were characterized.
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Yazar: Aradhika TRIPATHI
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Yazar: Rashmi AGGARWAL
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Yazar: Anita YADAV
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Bibtex @ { tbtkbiology139565, journal = {Turkish Journal of Biology}, issn = {1300-0152}, address = {TÜBİTAK}, year = {2014}, volume = {37}, pages = {606 - 613}, doi = {10.3906/biy-1301-16}, title = {Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat}, key = {cite}, author = {AGGARWAL, Rashmi and TRIPATHI, Aradhika and YADAV, Anita} }
APA TRIPATHI, A , AGGARWAL, R , YADAV, A . (2014). Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat. Turkish Journal of Biology, 37 (5), 606-613. DOI: 10.3906/biy-1301-16
MLA TRIPATHI, A , AGGARWAL, R , YADAV, A . "Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat". Turkish Journal of Biology 37 (2014): 606-613 <http://dergipark.gov.tr/tbtkbiology/issue/11690/139565>
Chicago TRIPATHI, A , AGGARWAL, R , YADAV, A . "Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat". Turkish Journal of Biology 37 (2014): 606-613
RIS TY - JOUR T1 - Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat AU - Aradhika TRIPATHI , Rashmi AGGARWAL , Anita YADAV Y1 - 2014 PY - 2014 N1 - doi: 10.3906/biy-1301-16 DO - 10.3906/biy-1301-16 T2 - Turkish Journal of Biology JF - Journal JO - JOR SP - 606 EP - 613 VL - 37 IS - 5 SN - 1300-0152-1303-6092 M3 - doi: 10.3906/biy-1301-16 UR - http://dx.doi.org/10.3906/biy-1301-16 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Biology Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat %A Aradhika TRIPATHI , Rashmi AGGARWAL , Anita YADAV %T Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat %D 2014 %J Turkish Journal of Biology %P 1300-0152-1303-6092 %V 37 %N 5 %R doi: 10.3906/biy-1301-16 %U 10.3906/biy-1301-16