Year 2015, Volume 45, Issue 6, Pages 1321 - 1328 2015-12-04

Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital
Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital

LANYING CUI [1] , JINGYING ZHAO [2] , JUAN LU [3]

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Background/aim: In recent years, the increase in the prevalence of carbapenem-resistant Enterobacter cloacae (CREC) has been significant and has become a global problem. The aim of this study is to characterize CREC extended spectrum β-lactamases (ESBLs) and CREC carbapenemase genes and evaluate the risk factors for CREC infections in a university hospital in China. Materials and methods: The identification and assessment of the antimicrobial susceptibility of CREC isolates were performed using a VITEK-2 compact system. Clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE). β-Lactamase genes were examined using polymerase chain reaction and nucleotide sequencing. Results: A total of 64 CREC isolates were included in the study. The rate of CREC infections significantly increased between 2009 and 2012. Among the CREC isolates, 39 (60.9%) isolates possessed ESBL genes, and 49 (76.6%) carried carbapenemase genes; blaSHV-12 and blaKPC-2 were the most common genes, respectively. PFGE showed that there were no obvious clonal associations between these CREC isolates, except for 3 isolates. A univariate analysis demonstrated that prior treatment with antibiotics, intravascular catheterization, urinary catheterization, mechanical ventilation, and parenteral nutrition were significant risk factors for CREC infections. Conclusion: The emergence and increase of CREC infections is worrisome. Effective measures should be taken to control the spread of CREC.
Background/aim: In recent years, the increase in the prevalence of carbapenem-resistant Enterobacter cloacae (CREC) has been significant and has become a global problem. The aim of this study is to characterize CREC extended spectrum β-lactamases (ESBLs) and CREC carbapenemase genes and evaluate the risk factors for CREC infections in a university hospital in China. Materials and methods: The identification and assessment of the antimicrobial susceptibility of CREC isolates were performed using a VITEK-2 compact system. Clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE). β-Lactamase genes were examined using polymerase chain reaction and nucleotide sequencing. Results: A total of 64 CREC isolates were included in the study. The rate of CREC infections significantly increased between 2009 and 2012. Among the CREC isolates, 39 (60.9%) isolates possessed ESBL genes, and 49 (76.6%) carried carbapenemase genes; blaSHV-12 and blaKPC-2 were the most common genes, respectively. PFGE showed that there were no obvious clonal associations between these CREC isolates, except for 3 isolates. A univariate analysis demonstrated that prior treatment with antibiotics, intravascular catheterization, urinary catheterization, mechanical ventilation, and parenteral nutrition were significant risk factors for CREC infections. Conclusion: The emergence and increase of CREC infections is worrisome. Effective measures should be taken to control the spread of CREC.
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Author: LANYING CUI

Author: JINGYING ZHAO

Author: JUAN LU

Bibtex @ { tbtkmedical149019, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {TUBITAK}, year = {2015}, volume = {45}, pages = {1321 - 1328}, doi = {}, title = {Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital}, key = {cite}, author = {CUI, LANYING and ZHAO, JINGYING and LU, JUAN} }
APA CUI, L , ZHAO, J , LU, J . (2015). Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital. Turkish Journal of Medical Sciences, 45 (6), 1321-1328. Retrieved from http://dergipark.gov.tr/tbtkmedical/issue/12391/149019
MLA CUI, L , ZHAO, J , LU, J . "Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital". Turkish Journal of Medical Sciences 45 (2015): 1321-1328 <http://dergipark.gov.tr/tbtkmedical/issue/12391/149019>
Chicago CUI, L , ZHAO, J , LU, J . "Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital". Turkish Journal of Medical Sciences 45 (2015): 1321-1328
RIS TY - JOUR T1 - Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital AU - LANYING CUI , JINGYING ZHAO , JUAN LU Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1321 EP - 1328 VL - 45 IS - 6 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2019 ER -
EndNote %0 Turkish Journal of Medical Sciences Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital %A LANYING CUI , JINGYING ZHAO , JUAN LU %T Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital %D 2015 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 45 %N 6 %R %U
ISNAD CUI, LANYING , ZHAO, JINGYING , LU, JUAN . "Molecular characteristics of extended spectrum ß-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital". Turkish Journal of Medical Sciences 45 / 6 (December 2015): 1321-1328.