Yıl 2015, Cilt 45, Sayı 6, Sayfalar 1345 - 1352 2015-12-04

PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*
PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*

JULİDE SEDEF GÖÇMEN [1] , NERİMAN ŞAHİNER [2] , MUKADDER KOÇAK [3] , ZEYNEP CEREN KARAHAN [4]

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Background/aim: Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls. Materials and methods: The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR. Results: Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg1397645907enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci. Conclusion: Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.
Background/aim: Staphylococcus aureus colonization is a determiner of disease activation in psoriasis patients. Here we evaluate the presence of genes encoding Panton-Valentine leukocidin (PVL), enterotoxins, TSST-1, exfoliative toxins, and the accessory gene regulatory locus by polymerase chain reaction (PCR) in S. aureus isolates obtained from healthy and diseased skin regions and anterior nares of psoriasis patients and healthy controls. Materials and methods: The presence of PVL and toxin genes was investigated, and agr typing was performed by PCR. Results: Eighteen of the isolated strains carried the sei, 1 carried the seb-sec, and 1 carried the seg1397645907enterotoxin gene. Eight of the strains carrying enterotoxin genes were isolated from nasal swabs, 6 from diseased skin swabs, and 4 from healthy skin swabs. None of the strains isolated from the control group carried the agr locus. On the other hand, 11 of the S. aureus strains isolated from the patients carried type 1, 7 carried type 1 + 3, 4 carried type 2, 4 carried type 3, and 1 carried type 1 + 2 agr loci. Conclusion: Enterotoxin production and the carried accessory gene regulatory locus may be important in the aggravation of psoriasis.
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Yazar: JULİDE SEDEF GÖÇMEN

Yazar: NERİMAN ŞAHİNER

Yazar: MUKADDER KOÇAK

Yazar: ZEYNEP CEREN KARAHAN

Bibtex @ { tbtkmedical149022, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {TÜBİTAK}, year = {2015}, volume = {45}, pages = {1345 - 1352}, doi = {}, title = {PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*}, key = {cite}, author = {KARAHAN, ZEYNEP CEREN and ŞAHİNER, NERİMAN and KOÇAK, MUKADDER and GÖÇMEN, JULİDE SEDEF} }
APA GÖÇMEN, J , ŞAHİNER, N , KOÇAK, M , KARAHAN, Z . (2015). PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*. Turkish Journal of Medical Sciences, 45 (6), 1345-1352. Retrieved from http://dergipark.gov.tr/tbtkmedical/issue/12391/149022
MLA GÖÇMEN, J , ŞAHİNER, N , KOÇAK, M , KARAHAN, Z . "PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*". Turkish Journal of Medical Sciences 45 (2015): 1345-1352 <http://dergipark.gov.tr/tbtkmedical/issue/12391/149022>
Chicago GÖÇMEN, J , ŞAHİNER, N , KOÇAK, M , KARAHAN, Z . "PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*". Turkish Journal of Medical Sciences 45 (2015): 1345-1352
RIS TY - JOUR T1 - PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients* AU - JULİDE SEDEF GÖÇMEN , NERİMAN ŞAHİNER , MUKADDER KOÇAK , ZEYNEP CEREN KARAHAN Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1345 EP - 1352 VL - 45 IS - 6 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2018 ER -
EndNote %0 Turkish Journal of Medical Sciences PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients* %A JULİDE SEDEF GÖÇMEN , NERİMAN ŞAHİNER , MUKADDER KOÇAK , ZEYNEP CEREN KARAHAN %T PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients* %D 2015 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 45 %N 6 %R %U
ISNAD GÖÇMEN, JULİDE SEDEF , ŞAHİNER, NERİMAN , KOÇAK, MUKADDER , KARAHAN, ZEYNEP CEREN . "PCR investigation of Panton-Valentine leukocidin, enterotoxin, exfoliative toxin,and agr genes in Staphylococcus aureus strains isolated from psoriasis patients*". Turkish Journal of Medical Sciences 45 / 6 (Aralık 2015): 1345-1352.