Yıl 2015, Cilt 45, Sayı 6, Sayfalar 1396 - 1402 2015-12-04

Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model
Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model

YAQUB HAJIAGHATABAR SANGROODI [1] , IRAJ RASOOLI [2] , SHAHRAM NAZARIAN [3] , WALEAD EBRAHIMIZADEH [4] , FATEMEH SEFID [5]

132 368

Background/aim: The uptake of the ferric-acinetobactin complex into the periplasmic space relies on the baumannii acinetobactin utilization (BauA) protein. BauA is composed of cork and the β-barrel domains. We constructed a recombinant protein from conserved antigenic domains of cork and the β-barrel of BauA to evaluate their immunogenic role in an animal model. Materials and methods: The selected bauA domains were amplified from a purified genome of Acinetobacter baumannii ATCC 19606. The domains were then cloned into pET28a and the proteins expressed in E. coli BL21 (DE3) were purified using nickel nitrilotriacetic acid chromatography. Mice and rabbits were immunized with an intraperitoneal injection of the recombinant BauA (rBauA). Results: The highest immune response was achieved after the third booster injection while hyperimmunity was achieved after the second booster injection in rabbits. Immunized mice challenged with live A. baumannii survived, whereas all unimmunized mice in the control group died after 24 h. Mice injected with 109 colony forming units of A. baumannii preincubated with pure immune rabbit sera survived. Bacterial cultures from mice spleen and liver specimens revealed the absence of bacterial growth in the immunized groups. Conclusion: The rBauA could be used as a prophylactic agent and further tests should be carried out to see if it may be useful in a clinical setting against A. baumannii infections.
Background/aim: The uptake of the ferric-acinetobactin complex into the periplasmic space relies on the baumannii acinetobactin utilization (BauA) protein. BauA is composed of cork and the β-barrel domains. We constructed a recombinant protein from conserved antigenic domains of cork and the β-barrel of BauA to evaluate their immunogenic role in an animal model. Materials and methods: The selected bauA domains were amplified from a purified genome of Acinetobacter baumannii ATCC 19606. The domains were then cloned into pET28a and the proteins expressed in E. coli BL21 (DE3) were purified using nickel nitrilotriacetic acid chromatography. Mice and rabbits were immunized with an intraperitoneal injection of the recombinant BauA (rBauA). Results: The highest immune response was achieved after the third booster injection while hyperimmunity was achieved after the second booster injection in rabbits. Immunized mice challenged with live A. baumannii survived, whereas all unimmunized mice in the control group died after 24 h. Mice injected with 109 colony forming units of A. baumannii preincubated with pure immune rabbit sera survived. Bacterial cultures from mice spleen and liver specimens revealed the absence of bacterial growth in the immunized groups. Conclusion: The rBauA could be used as a prophylactic agent and further tests should be carried out to see if it may be useful in a clinical setting against A. baumannii infections.
  • Eliopoulos GM, Maragakis LL, Perl TM. Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clin Infect Dis 2008; 46: 1254–1263.
  • Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 2008; 21: 538–582.
  • Dijkshoorn L, Nemec A, Seifert H. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol 2007; 5: 939–951.
  • Valencia R, Arroyo LA, Conde M, Aldana JM, Torres MJ, Garnacho‐Montero J, Cisneros JM, Ortíz C, Pachón J, Aznar J. Nosocomial outbreak of infection with pan–drug‐resistant Acinetobacter baumannii in a tertiary care university hospital. Infect Control Hosp Epidemiol 2009; 30: 257–263.
  • Aydın M, Tevfik M, Korkut O, Oldaçay M. Antibiotic resistance profile of Acinetobacter strains isolated from patients in the intensive care unit: a surveillance study of four years. Mediterr J Infect Microb Antimicrob 2013; 2: 13.
  • Gaddy JA, Arivett BA, McConnell MJ, López-Rojas R, Pachón J, Actis LA. Role of acinetobactin-mediated iron acquisition functions in the interaction of Acinetobacter baumannii strain ATCC 19606T with human lung epithelial cells, Galleria mellonella caterpillars, and mice. Infect Immun 2012; 80: 1015–1024.
  • Andrews SC, Robinson AK, Rodríguez‐Quiñones F. Bacterial iron homeostasis. FEMS Microbiol Rev 2003; 27: 215–237.
  • Ratledge C, Dover LG. Iron metabolism in pathogenic bacteria. Ann Rev Microbiol 2000; 54: 881–941.
  • Skaar EP. The battle for iron between bacterial pathogens and their vertebrate hosts. PLoS Pathog 2010; 6: e1000949.
  • Ganz T. Iron in innate immunity: starve the invaders. Curr Opin Immunol 2009; 21: 63–67.
  • Ong ST, Shan Ho JZ, Ho B, Ding JL. Iron-withholding strategy in innate immunity. Immunobiology 2006; 211: 295–314.
  • Sandrini SM, Shergill R, Woodward J, Muralikuttan R, Haigh RD, Lyte M, Freestone PP. Elucidation of the mechanism by which catecholamine stress hormones liberate iron from the innate immune defense proteins transferrin and lactoferrin. J Bacteriol 2010; 192: 587–594.
  • Krewulak KD, Vogel HJ. Structural biology of bacterial iron uptake. Biochim Biophys Acta 2008; 1778: 1781–1804.
  • Santander J, Golden G, Wanda SY, Curtiss R 3rd. Fur-regulated iron uptake system of Edwardsiella ictaluri and its influence on pathogenesis and immunogenicity in the catfish host. Infect Immun 2012; 80: 2689–2703.
  • Vallenet D, Nordmann P, Barbe V, Poirel L, Mangenot S, Bataille E, Dossat C, Gas S, Kreimeyer A, Lenoble P. Comparative analysis of Acinetobacters: three genomes for three lifestyles. PLoS One 2008; 3: e1805.
  • Mihara K, Tanabe T, Yamakawa Y, Funahashi T, Nakao H, Narimatsu S, Yamamoto S. Identification and transcriptional organization of a gene cluster involved in biosynthesis and transport of acinetobactin, a siderophore produced by Acinetobacter baumannii ATCC 19606T. Microbiology 2004; 150: 2587–2597.
  • Zimbler DL, Penwell WF, Gaddy JA, Menke SM, Tomaras AP, Connerly PL, Actis LA. Iron acquisition functions expressed by the human pathogen Acinetobacter baumannii. Biometals 2009; 22: 23–32.
  • Sefid F, Rasooli I, Jahangiri A. In silico determination and validation of baumannii acinetobactin utilization: a structure and ligand binding site. BioMed Research International 2013; 2013: 172784.
  • Zimbler DL, Arivett BA, Beckett AC, Menke SM, Actis LA. Functional features of TonB energy transduction systems of Acinetobacter baumannii. Infect Immun 2013; 81: 3382–3394.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248– 254.
  • Cooper AJ, Pinto JT, Callery PS. Reversible and irreversible protein glutathionylation: biological and clinical aspects. Expert Opin Drug Metab Toxicol 2011; 7: 891–910.
  • Eijkelkamp BA, Hassan KA, Paulsen IT, Brown MH. Investigation of the human pathogen Acinetobacter baumannii under iron limiting conditions. BMC Genomics 2011; 12: 126.
  • Schwyn B, Neilands J. Universal chemical assay for the detection and determination of siderophores. Anal Biochem 1987; 160: 47–56.
  • Stiles BG, Sexton FW. Immunoreactivity, epitope mapping and protection studies with anti-conotoxin GI sera and various conotoxins. Toxicon 1992; 30: 36–7377.
  • Brenner DJ, Krieg NR, Staley J, Garrity G, editors. Bergey’s Manual of Systematic Bacteriology, Vol. 2: The Proteobacteria. East Lansing, MI, USA: Springer; 2005.
  • García-Garmendia JL, Ortiz-Leyba C, Garnacho-Montero J, Jiménez-Jiménez FJ, Pérez-Paredes C, Barrero-Almodóvar AE, Miner MG. Risk factors for Acinetobacter baumannii nosocomial bacteremia in critically ill patients: a cohort study. Clin Infect Dis 2001; 33: 939–946.
  • Bakherad H, Gargari SLM, Rasooli I, RajabiBazl M, Mohammadi M, Ebrahimizadeh W, Ardakani LS, Zare H. In vivo neutralization of botulinum neurotoxins serotype E with heavy-chain camelid antibodies (VHH). Mol Biotechnol 2013; 55: 159–167.
  • Ebrahimizadeh W, Gargari SM, Rajabibazl M, Ardekani LS, Zare H, Bakherad H. Isolation and characterization of protective anti-LPS nanobody against V. cholerae O1 recognizing Inaba and Ogawa serotypes. Appl Microbiol Biotechnol 2013; 97: 4457–4466.
  • Malekshahi ZV, Gargari SLM, Rasooli I, Ebrahimizadeh W. Treatment of Helicobacter pylori infection in mice with oral administration of egg yolk-driven anti-UreC immunoglobulin. Microb Pathog 2011; 51: 366–372.
  • Kaneshige T, Yaguchi K, Ohgitani T. Siderophore receptor IroN is an important protective antigen against Salmonella infection in chickens. Avian Dis 2009; 53: 563–567.
  • Larrie-Bagha SM, Rasooli I, Mousavi-Gargari SL, Rasooli Z, Nazarian S. Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157. Iranian J Microbiol 2013; 5: 113.
  • Marsh JW, O’Leary MM, Shutt KA, Harrison LH. Deletion of fetA gene sequences in serogroup B and C Neisseria meningitidis isolates. J Clin Microbiol 2007; 45: 1333–1335.
  • Fattahian Y, Rasooli I, Mousavi Gargari SL, Rahbar MR, Darvish Alipour Astaneh S, Amani J. Protection against Acinetobacter baumannii infection via its functional deprivation of biofilm associated protein (Bap). Microb Pathog 2011; 51: 402–406.
  • Goel VK, Kapil A. Monoclonal antibodies against the iron regulated outer membrane proteins of Acinetobacter baumannii are bactericidal. BMC Microbiol 2001; 1: 16.
Birincil Dil tr
Konular
Dergi Bölümü Makaleler
Yazarlar

Yazar: YAQUB HAJIAGHATABAR SANGROODI

Yazar: IRAJ RASOOLI

Yazar: SHAHRAM NAZARIAN

Yazar: WALEAD EBRAHIMIZADEH

Yazar: FATEMEH SEFID

Bibtex @ { tbtkmedical149029, journal = {Turkish Journal of Medical Sciences}, issn = {1300-0144}, eissn = {1303-6165}, address = {TÜBİTAK}, year = {2015}, volume = {45}, pages = {1396 - 1402}, doi = {}, title = {Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model}, key = {cite}, author = {SANGROODI, YAQUB HAJIAGHATABAR and EBRAHIMIZADEH, WALEAD and NAZARIAN, SHAHRAM and RASOOLI, IRAJ and SEFID, FATEMEH} }
APA SANGROODI, Y , RASOOLI, I , NAZARIAN, S , EBRAHIMIZADEH, W , SEFID, F . (2015). Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model. Turkish Journal of Medical Sciences, 45 (6), 1396-1402. Retrieved from http://dergipark.gov.tr/tbtkmedical/issue/12391/149029
MLA SANGROODI, Y , RASOOLI, I , NAZARIAN, S , EBRAHIMIZADEH, W , SEFID, F . "Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model". Turkish Journal of Medical Sciences 45 (2015): 1396-1402 <http://dergipark.gov.tr/tbtkmedical/issue/12391/149029>
Chicago SANGROODI, Y , RASOOLI, I , NAZARIAN, S , EBRAHIMIZADEH, W , SEFID, F . "Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model". Turkish Journal of Medical Sciences 45 (2015): 1396-1402
RIS TY - JOUR T1 - Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model AU - YAQUB HAJIAGHATABAR SANGROODI , IRAJ RASOOLI , SHAHRAM NAZARIAN , WALEAD EBRAHIMIZADEH , FATEMEH SEFID Y1 - 2015 PY - 2015 N1 - DO - T2 - Turkish Journal of Medical Sciences JF - Journal JO - JOR SP - 1396 EP - 1402 VL - 45 IS - 6 SN - 1300-0144-1303-6165 M3 - UR - Y2 - 2018 ER -
EndNote %0 Turkish Journal of Medical Sciences Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model %A YAQUB HAJIAGHATABAR SANGROODI , IRAJ RASOOLI , SHAHRAM NAZARIAN , WALEAD EBRAHIMIZADEH , FATEMEH SEFID %T Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model %D 2015 %J Turkish Journal of Medical Sciences %P 1300-0144-1303-6165 %V 45 %N 6 %R %U
ISNAD SANGROODI, YAQUB HAJIAGHATABAR , RASOOLI, IRAJ , NAZARIAN, SHAHRAM , EBRAHIMIZADEH, WALEAD , SEFID, FATEMEH . "Immunogenicity of conserved cork and ß-barrel domains of baumannii acinetobactin utilization protein in an animal model". Turkish Journal of Medical Sciences 45 / 6 (Aralık 2015): 1396-1402.