Yıl 2014, Cilt 38, Sayı 3, Sayfalar 225 - 229 2014-05-18

Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey
Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey

Özgecan KORKMAZ AĞAOĞLU [1] , Mustafa SAATCI [2] , Özkan ELMAZ [3] , Mehmet ÇOLAK [4] , Mesih KOCAMÜFTÜOĞLU [5] , Emel ZEYTÜNLÜ [6]

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Alpha-lactalbumin plays a key role in lactose synthesis in the mammary glands of domestic animals. This study evaluated Hair (188), Saanen (82), Kilis (63), and Honamli (183) goat breeds, with the primary goal of investigating DNA polymorphism of Mva I RFLP at exon 3 of the alpha-lactalbumin gene. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotype a total of 516 goats for Mva I polymorphism of alpha-lactalbumin. In the breeds that were studied, digestion of the amplification product with the Mva I restriction enzyme revealed 2 alleles (A1 and A2) and 3 genotypes (A1A1, A1A2, and A2A2). Allelic frequencies for Hair, Honamli, Saanen, and Kilis breeds were found to be 0.87, 0.92, 0.87, and 0.85, respectively, for the A1 allele and 0.13, 0.08, 0.13, and 0.15, respectively, for the A2 allele. None of the studied breeds exhibited significant deviation from Hardy–Weinberg equilibrium. Consequently, this study shed light on the polymorphism of alpha-lactalbumin in 4 goat breeds. Another finding of this study was the presence of genetic polymorphism at the alpha-lactalbumin gene in Kilis and Honamli goat breeds, a fact that was not known previously.
Alpha-lactalbumin plays a key role in lactose synthesis in the mammary glands of domestic animals. This study evaluated Hair (188), Saanen (82), Kilis (63), and Honamli (183) goat breeds, with the primary goal of investigating DNA polymorphism of Mva I RFLP at exon 3 of the alpha-lactalbumin gene. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotype a total of 516 goats for Mva I polymorphism of alpha-lactalbumin. In the breeds that were studied, digestion of the amplification product with the Mva I restriction enzyme revealed 2 alleles (A1 and A2) and 3 genotypes (A1A1, A1A2, and A2A2). Allelic frequencies for Hair, Honamli, Saanen, and Kilis breeds were found to be 0.87, 0.92, 0.87, and 0.85, respectively, for the A1 allele and 0.13, 0.08, 0.13, and 0.15, respectively, for the A2 allele. None of the studied breeds exhibited significant deviation from Hardy–Weinberg equilibrium. Consequently, this study shed light on the polymorphism of alpha-lactalbumin in 4 goat breeds. Another finding of this study was the presence of genetic polymorphism at the alpha-lactalbumin gene in Kilis and Honamli goat breeds, a fact that was not known previously.
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Yazar: Özgecan KORKMAZ AĞAOĞLU

Yazar: Mustafa SAATCI

Yazar: Özkan ELMAZ

Yazar: Mehmet ÇOLAK

Yazar: Mesih KOCAMÜFTÜOĞLU

Yazar: Emel ZEYTÜNLÜ

Bibtex @ { tbtkveterinary150449, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {TÜBİTAK}, year = {2014}, volume = {38}, pages = {225 - 229}, doi = {10.3906/sag-1304-122}, title = {Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey}, key = {cite}, author = {ELMAZ, Özkan and AĞAOĞLU, Özgecan KORKMAZ and SAATCI, Mustafa and KOCAMÜFTÜOĞLU, Mesih and ÇOLAK, Mehmet and ZEYTÜNLÜ, Emel} }
APA AĞAOĞLU, Ö , SAATCI, M , ELMAZ, Ö , ÇOLAK, M , KOCAMÜFTÜOĞLU, M , ZEYTÜNLÜ, E . (2014). Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey. Turkish Journal of Veterinary and Animal Sciences, 38 (3), 225-229. DOI: 10.3906/sag-1304-122
MLA AĞAOĞLU, Ö , SAATCI, M , ELMAZ, Ö , ÇOLAK, M , KOCAMÜFTÜOĞLU, M , ZEYTÜNLÜ, E . "Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey". Turkish Journal of Veterinary and Animal Sciences 38 (2014): 225-229 <http://dergipark.gov.tr/tbtkveterinary/issue/12490/150449>
Chicago AĞAOĞLU, Ö , SAATCI, M , ELMAZ, Ö , ÇOLAK, M , KOCAMÜFTÜOĞLU, M , ZEYTÜNLÜ, E . "Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey". Turkish Journal of Veterinary and Animal Sciences 38 (2014): 225-229
RIS TY - JOUR T1 - Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey AU - Özgecan KORKMAZ AĞAOĞLU , Mustafa SAATCI , Özkan ELMAZ , Mehmet ÇOLAK , Mesih KOCAMÜFTÜOĞLU , Emel ZEYTÜNLÜ Y1 - 2014 PY - 2014 N1 - doi: 10.3906/sag-1304-122 DO - 10.3906/sag-1304-122 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 225 EP - 229 VL - 38 IS - 3 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/sag-1304-122 UR - http://dx.doi.org/10.3906/sag-1304-122 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey %A Özgecan KORKMAZ AĞAOĞLU , Mustafa SAATCI , Özkan ELMAZ , Mehmet ÇOLAK , Mesih KOCAMÜFTÜOĞLU , Emel ZEYTÜNLÜ %T Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 38 %N 3 %R doi: 10.3906/sag-1304-122 %U 10.3906/sag-1304-122
ISNAD AĞAOĞLU, Özgecan KORKMAZ , SAATCI, Mustafa , ELMAZ, Özkan , ÇOLAK, Mehmet , KOCAMÜFTÜOĞLU, Mesih , ZEYTÜNLÜ, Emel . "Mva I PCR-RFLP identifies single nucleotide polymorphism of the alpha-lactalbumin gene in some goat breeds reared in Turkey". Turkish Journal of Veterinary and Animal Sciences 38 / 3 (Mayıs 2014): 225-229. http://dx.doi.org/10.3906/sag-1304-122