Yıl 2014, Cilt 38, Sayı 3, Sayfalar 325 - 330 2014-05-18

NMR-based metabolomic evaluation in dairy cows with displaced abomasum
NMR-based metabolomic evaluation in dairy cows with displaced abomasum

Abdullah BAŞOĞLU [1] , Nuri BAŞPINAR [2] , Alparslan COŞKUN [3]

159 377

This study is the first to evaluate nuclear magnetic resonance (NMR)-based metabolomics in cows with displaced abomasum (DA), which is an internationally recognized problem in dairy cows. Some biochemical parameters have been used for monitoring DA. However, to date, few data have been available on the blood metabolomic profile of dairy cows. Forty Holstein multiparous cows with DA (30 left, 10 right) and 10 clinically healthy Holstein multiparous cows were the subjects of the study. All the animals had similar dry matter intake. An NMR-based metabolomics approach and hematological and biochemical analyses were performed. Some changes in biochemical parameters were observed between the groups. Among the cows with left displaced abomasum (LDA), 10 were associated with concomitant ketosis. Disease periods between the 2 DA groups were different. The metabolites identified and quantified by NMR analysis were valine, 3 beta-hydroxybutyrate (BHB), alanine, glutamine, glutamate, and succinate. The last of these was significantly decreased in cows with right displaced abomasum (RDA). Glutamine, glutamate, and 3 BHB levels were significantly different between DA groups. There was a positive correlation between BHB and valine, glutamine, and glutamate in the LDA group. Overall, this work suggests that the additional information obtained by NMR-based metabolomics evaluation may contribute to assessing the metabolic status of cows with DA.
This study is the first to evaluate nuclear magnetic resonance (NMR)-based metabolomics in cows with displaced abomasum (DA), which is an internationally recognized problem in dairy cows. Some biochemical parameters have been used for monitoring DA. However, to date, few data have been available on the blood metabolomic profile of dairy cows. Forty Holstein multiparous cows with DA (30 left, 10 right) and 10 clinically healthy Holstein multiparous cows were the subjects of the study. All the animals had similar dry matter intake. An NMR-based metabolomics approach and hematological and biochemical analyses were performed. Some changes in biochemical parameters were observed between the groups. Among the cows with left displaced abomasum (LDA), 10 were associated with concomitant ketosis. Disease periods between the 2 DA groups were different. The metabolites identified and quantified by NMR analysis were valine, 3 beta-hydroxybutyrate (BHB), alanine, glutamine, glutamate, and succinate. The last of these was significantly decreased in cows with right displaced abomasum (RDA). Glutamine, glutamate, and 3 BHB levels were significantly different between DA groups. There was a positive correlation between BHB and valine, glutamine, and glutamate in the LDA group. Overall, this work suggests that the additional information obtained by NMR-based metabolomics evaluation may contribute to assessing the metabolic status of cows with DA.
  • Mulligan FJ, Doherty ML. Production diseases of the transition cow. Vet J 2008; 176: 3–9.
  • Doll K, Sickinger M, Seeger T. New aspects in the pathogenesis of abomasal displacement. Vet J 2009; 181: 90–96.
  • Sen I, Ok M, Coskun A. The Level of serum ionised calcium, aspartate aminotransferase, insulin, glucose, betahydroxybutyrate concentrations and blood gas parameters in cows with left displacement of abomasums. Pol J Vet Sci 2006; 9: 227–232.
  • Parish SM. Diseases of dairy animal. Non-infectious diseases: displaced abomasum. In: Fuquay JW, editor. Encyclopedia of Dairy Sciences. 2nd ed. Elsevier Inc., St Louis, MO, USA: Saunders; 2011. pp. 212–216.
  • Anderson DE. Pathophysiology of displacement of the abomasum in cattle. In: Anderson DE, editor. Food Animal Practice, Chapter 12. 5th ed. Elsevier Inc., St Louis, MO, USA: Saunders; 2009. pp. 35–40.
  • Seifi HA, Leblanc SJ, Leslie KE, Duffield TF. Metabolic predictors of post-partum disease and culling risk in dairy cattle. Vet J 2011; 188: 216–220.
  • Guzelbektes H, Sen I, Ok M, Constable PD, Boydak M, Coskun A. Serum amyloid A and haptoglobin concentrations and liver fat percentage in lactating dairy cows with abomasal displacement. J Vet Intern Med 2010; 24: 213–219.
  • Maden M, Ozturk AS, Bulbul A, Avci GE, Yazar E. Acute-phase proteins, oxidative stress and enzyme activities of blood serum and peritoneal fluid in cattle with abomasal displacement. J Vet Intern Med 2012; 26: 1470–1475.
  • Grosche A, Fürll M, Wittek T. Peritoneal fluid analysis in dairy cows with left displaced abomasum and abomasal volvulus. Vet Rec 2012; 170: 413.
  • Xia J, Broadhurst DI, Wilson M, Wishart DS. Translational biomarker discovery in clinical metabolomics: an introductory tutorial. Metabolomics 2013; 9: 280–299.
  • Serkova NJ, Brown MS. Quantitative analysis in magnetic resonance spectroscopy: from metabolic profiling to in vivo biomarkers. Bioanalysis 2012; 4: 321–341.
  • Stringer KA, Serkova NJ, Karnovsky A, Guire K, Paine R, Standiford TJ. Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis. Am J Physiol Lung Cell Mol Physiol 2011; 300: L4–L11.
  • Constable P, Grünberg W, Staufenbiel R, Stämpfli HR. Clinicopathologic variables associated with hypokalemia in lactating dairy cows with abomasal displacement or volvulus. JAVMA 2013; 242: 826–835.
  • Stengärde L, Holtenius K, Tråvén M, Hultgren J, Niskanen R, Emanuelson U. Blood profiles in dairy cows with displaced abomasum. J Dairy Sci 2010; 93: 4691–4699.
  • Van Winden SC, Jorritsma R, Müller KE, Noordhuizen JP. Feed intake, milk yield, and metabolic parameters prior to left displaced abomasum in dairy cows. J Dairy Sci 2003; 86: 1465–1471.
  • Leblanc S. Monitoring metabolic health of dairy cattle in the transition period. J Reprod Dev 2010; 56: Suppl: 29–35.
  • LeBlanc SJ, Leslie KE, Duffield TF. Metabolic predictors of displaced abomasum in dairy cattle. J Dairy Sci 2005; 88: 159–170.
  • Ospina PA, McArt JA, Overton TR, Stokol T, Nydam DV. Using nonesterified fatty acids and b-hydroxybutyrate concentrations during the transition period for herd-level monitoring of increased risk of disease and decreased reproductive and milking performance. Vet Clin North Am Food Anim Pract 2013; 29: 387–412.
  • Stengarde L, Tråvén M, Emanuelson U, Holtenius K, Hultgren J, Niskanen R. Metabolic profiles in five high-producing Swedish dairy herds with a history of abomasal displacement and ketosis. Acta Vet Scand 2008; 50: 31–42.
  • Sevinc M, Ok M, Basoglu A. Liver function in dairy cows with abomasal displacement. Revue Méd Vét 2002; 153: 477–480.
  • Civelek T, Sevinc M, Boydak M, Basoglu A. Serum apolipoprotein B100 concentrations in dairy cows with left displaced abomasum. Revue Méd Vét 2006; 157: 361–365.
  • Klein MS, Buttchereit N, Miemczyk SP, Immervoll AK, Louis C, Wiedemann S Junge W, Thaller G, Oefner PJ, Gronwald W. NMR metabolomic analysis of dairy cows reveals milk glycerophosphocholine to phosphocholine ratio as prognostic biomarker for risk of ketosis. Proteome Res 2012; 11: 1373–1381.
  • Ilves A, Harzia H, Ling K, Ots M, Soomets U, Kilk K. Alterations in milk and blood metabolomes during the first months of lactation in dairy cows. J Dairy Sci 2012; 95: 5788–5797.
  • Hamana M, Ohtsuka H, Oikawa M, Kawamura S. Blood free amino acids in the postpartum dairy cattle with left displaced abomasums. J Vet Med Sci 2010; 72: 1355–1358.
  • Martínez-Granados B, Morales JM, Rodrigo JM, Del Olmo J, Serra MA, Ferrández A, Celda B, Monleón D. Metabolic profile of chronic liver disease by NMR spectroscopy of human biopsies. Int J Mol Med 2011; 27: 111–117.
Birincil Dil tr
Konular
Dergi Bölümü Makaleler
Yazarlar

Yazar: Abdullah BAŞOĞLU

Yazar: Nuri BAŞPINAR

Yazar: Alparslan COŞKUN

Bibtex @ { tbtkveterinary150464, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {TÜBİTAK}, year = {2014}, volume = {38}, pages = {325 - 330}, doi = {10.3906/sag-1303-128}, title = {NMR-based metabolomic evaluation in dairy cows with displaced abomasum}, key = {cite}, author = {BAŞPINAR, Nuri and COŞKUN, Alparslan and BAŞOĞLU, Abdullah} }
APA BAŞOĞLU, A , BAŞPINAR, N , COŞKUN, A . (2014). NMR-based metabolomic evaluation in dairy cows with displaced abomasum. Turkish Journal of Veterinary and Animal Sciences, 38 (3), 325-330. DOI: 10.3906/sag-1303-128
MLA BAŞOĞLU, A , BAŞPINAR, N , COŞKUN, A . "NMR-based metabolomic evaluation in dairy cows with displaced abomasum". Turkish Journal of Veterinary and Animal Sciences 38 (2014): 325-330 <http://dergipark.gov.tr/tbtkveterinary/issue/12490/150464>
Chicago BAŞOĞLU, A , BAŞPINAR, N , COŞKUN, A . "NMR-based metabolomic evaluation in dairy cows with displaced abomasum". Turkish Journal of Veterinary and Animal Sciences 38 (2014): 325-330
RIS TY - JOUR T1 - NMR-based metabolomic evaluation in dairy cows with displaced abomasum AU - Abdullah BAŞOĞLU , Nuri BAŞPINAR , Alparslan COŞKUN Y1 - 2014 PY - 2014 N1 - doi: 10.3906/sag-1303-128 DO - 10.3906/sag-1303-128 T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 325 EP - 330 VL - 38 IS - 3 SN - 1300-0128-1303-6181 M3 - doi: 10.3906/sag-1303-128 UR - http://dx.doi.org/10.3906/sag-1303-128 Y2 - 2018 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences NMR-based metabolomic evaluation in dairy cows with displaced abomasum %A Abdullah BAŞOĞLU , Nuri BAŞPINAR , Alparslan COŞKUN %T NMR-based metabolomic evaluation in dairy cows with displaced abomasum %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 38 %N 3 %R doi: 10.3906/sag-1303-128 %U 10.3906/sag-1303-128
ISNAD BAŞOĞLU, Abdullah , BAŞPINAR, Nuri , COŞKUN, Alparslan . "NMR-based metabolomic evaluation in dairy cows with displaced abomasum". Turkish Journal of Veterinary and Animal Sciences 38 / 3 (Mayıs 2014): 325-330. http://dx.doi.org/10.3906/sag-1303-128