Cilt 36, Sayı 3, Sayfalar 305 - 310 2014-05-18

Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR
Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR

Seran TEMELLİ [1] , Ayşegül EYİGÖR* [2] , Şahsene ANAR [3]

70 309

This study aimed to determine the prevalence of Escherichia coli O157 in retail red meat and meat products with the Vitek Immunodiagnostic Assay System, including H7 Escherichia coli phage technology (VIDAS ECPT), and a real-time polymerase chain reaction system (LightCycler PCR; LCPCR). A total of 106 red meat and meat product samples were analyzed with VIDAS ECPT and LCPCR. VIDAS ECPT presumptive positive samples were subjected to VIDAS Immuno Concentration E. coli O157, followed by culture and serology. Among the 27 out of 72 (37.50%) red meat samples and 3 out of 34 (8.82%) red meat products that tested positive by VIDAS ECPT, 5.55% and 0.00% were confirmed positive for E. coli O157 (but not H7), respectively. Red meat and red meat product samples were 73.61% and 20.58% positive by LCPCR. The 5.55% prevalence of E. coli O157 in red meats poses a significant risk for consumers and indicates insufficient hygiene management both at the farm and during the slaughtering and meat handling processes in Turkey. This is the first report on the detection of E. coli O157 by VIDAS ECPT and LCPCR in naturally contaminated red meat and meat products.
This study aimed to determine the prevalence of Escherichia coli O157 in retail red meat and meat products with the Vitek Immunodiagnostic Assay System, including H7 Escherichia coli phage technology (VIDAS ECPT), and a real-time polymerase chain reaction system (LightCycler PCR; LCPCR). A total of 106 red meat and meat product samples were analyzed with VIDAS ECPT and LCPCR. VIDAS ECPT presumptive positive samples were subjected to VIDAS Immuno Concentration E. coli O157, followed by culture and serology. Among the 27 out of 72 (37.50%) red meat samples and 3 out of 34 (8.82%) red meat products that tested positive by VIDAS ECPT, 5.55% and 0.00% were confirmed positive for E. coli O157 (but not H7), respectively. Red meat and red meat product samples were 73.61% and 20.58% positive by LCPCR. The 5.55% prevalence of E. coli O157 in red meats poses a significant risk for consumers and indicates insufficient hygiene management both at the farm and during the slaughtering and meat handling processes in Turkey. This is the first report on the detection of E. coli O157 by VIDAS ECPT and LCPCR in naturally contaminated red meat and meat products.
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Yazar: Seran TEMELLİ
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Yazar: Ayşegül EYİGÖR*
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Yazar: Şahsene ANAR
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Bibtex @ { tbtkveterinary150718, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, address = {TÜBİTAK}, year = {2014}, volume = {36}, pages = {305 - 310}, doi = {}, title = {Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR}, language = {en}, key = {cite}, author = {ANAR, Şahsene and TEMELLİ, Seran and EYİGÖR*, Ayşegül} } @ { tbtkveterinary150718, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, address = {TÜBİTAK}, year = {2014}, volume = {36}, pages = {305 - 310}, doi = {}, title = {Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR}, language = {tr}, key = {cite}, author = {ANAR, Şahsene and TEMELLİ, Seran and EYİGÖR*, Ayşegül} }
APA TEMELLİ, S , EYİGÖR*, A , ANAR, Ş . (2014). Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR. Turkish Journal of Veterinary and Animal Sciences, 36 (3), 305-310. Retrieved from http://dergipark.gov.tr/tbtkveterinary/issue/12502/150718
MLA TEMELLİ, S , EYİGÖR*, A , ANAR, Ş . "Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR". Turkish Journal of Veterinary and Animal Sciences 36 (2014): 305-310 <http://dergipark.gov.tr/tbtkveterinary/issue/12502/150718>
Chicago TEMELLİ, S , EYİGÖR*, A , ANAR, Ş . "Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR". Turkish Journal of Veterinary and Animal Sciences 36 (2014): 305-310
RIS TY - JOUR T1 - Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR AU - Seran TEMELLİ , Ayşegül EYİGÖR* , Şahsene ANAR Y1 - 2014 PY - 2014 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 305 EP - 310 VL - 36 IS - 3 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2017 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR %A Seran TEMELLİ , Ayşegül EYİGÖR* , Şahsene ANAR %T Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 36 %N 3 %R %U