Year 2012, Volume 36, Issue 3, Pages 311 - 318 2014-05-18

The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia
The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

Mutlu KÜÇÜK [1] , Mehmet KAYA [2] , Nurcan ORHAN [3] , Oğuzhan EKİZOĞLU [4] , Erol ARSLAN [5] , Nadir ARICAN [6] , R.Candan GÜRSES [7] , İmdat ELMAS [8] , Rivaze KALAYCI [9] , Bülent AHISHALI [10]

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The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
Primary Language tr
Subjects
Journal Section Articles
Authors

Author: Mutlu KÜÇÜK

Author: Mehmet KAYA

Author: Nurcan ORHAN

Author: Oğuzhan EKİZOĞLU

Author: Erol ARSLAN

Author: Nadir ARICAN

Author: R.Candan GÜRSES

Author: İmdat ELMAS

Author: Rivaze KALAYCI

Author: Bülent AHISHALI

Bibtex @ { tbtkveterinary150719, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {TUBITAK}, year = {2014}, volume = {36}, pages = {311 - 318}, doi = {10.3906/sag-1203-43}, title = {The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia}, key = {cite}, author = {ELMAS, İmdat and KALAYCI, Rivaze and GÜRSES, R.Candan and EKİZOĞLU, Oğuzhan and ORHAN, Nurcan and ARICAN, Nadir and KÜÇÜK, Mutlu and KAYA, Mehmet and ARSLAN, Erol and AHISHALI, Bülent} }
APA KÜÇÜK, M , KAYA, M , ORHAN, N , EKİZOĞLU, O , ARSLAN, E , ARICAN, N , GÜRSES, R , ELMAS, İ , KALAYCI, R , AHISHALI, B . (2014). The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia. Turkish Journal of Veterinary and Animal Sciences, 36 (3), 311-318. Retrieved from http://dergipark.gov.tr/tbtkveterinary/issue/12502/150719
MLA KÜÇÜK, M , KAYA, M , ORHAN, N , EKİZOĞLU, O , ARSLAN, E , ARICAN, N , GÜRSES, R , ELMAS, İ , KALAYCI, R , AHISHALI, B . "The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia". Turkish Journal of Veterinary and Animal Sciences 36 (2014): 311-318 <http://dergipark.gov.tr/tbtkveterinary/issue/12502/150719>
Chicago KÜÇÜK, M , KAYA, M , ORHAN, N , EKİZOĞLU, O , ARSLAN, E , ARICAN, N , GÜRSES, R , ELMAS, İ , KALAYCI, R , AHISHALI, B . "The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia". Turkish Journal of Veterinary and Animal Sciences 36 (2014): 311-318
RIS TY - JOUR T1 - The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia AU - Mutlu KÜÇÜK , Mehmet KAYA , Nurcan ORHAN , Oğuzhan EKİZOĞLU , Erol ARSLAN , Nadir ARICAN , R.Candan GÜRSES , İmdat ELMAS , Rivaze KALAYCI , Bülent AHISHALI Y1 - 2014 PY - 2014 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 311 EP - 318 VL - 36 IS - 3 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2018 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia %A Mutlu KÜÇÜK , Mehmet KAYA , Nurcan ORHAN , Oğuzhan EKİZOĞLU , Erol ARSLAN , Nadir ARICAN , R.Candan GÜRSES , İmdat ELMAS , Rivaze KALAYCI , Bülent AHISHALI %T The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 36 %N 3 %R %U