Cilt 36, Sayı 3, Sayfalar 311 - 318 2014-05-18

The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia
The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

Mutlu KÜÇÜK [1] , Mehmet KAYA [2] , Nurcan ORHAN [3] , Oğuzhan EKİZOĞLU [4] , Erol ARSLAN [5] , Nadir ARICAN [6] , R.Candan GÜRSES [7] , İmdat ELMAS [8] , Rivaze KALAYCI [9] , Bülent AHISHALI [10]

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The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
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Yazar: Mutlu KÜÇÜK
E-posta:

Yazar: Mehmet KAYA
E-posta:

Yazar: Nurcan ORHAN
E-posta:

Yazar: Oğuzhan EKİZOĞLU
E-posta:

Yazar: Erol ARSLAN
E-posta:

Yazar: Nadir ARICAN
E-posta:

Yazar: R.Candan GÜRSES
E-posta:

Yazar: İmdat ELMAS
E-posta:

Yazar: Rivaze KALAYCI
E-posta:

Yazar: Bülent AHISHALI
E-posta:

Bibtex @ { tbtkveterinary150719, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, address = {TÜBİTAK}, year = {2014}, volume = {36}, pages = {311 - 318}, doi = {10.3906/sag-1203-43}, title = {The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia}, language = {en}, key = {cite}, author = {ELMAS, İmdat and KALAYCI, Rivaze and GÜRSES, R.Candan and EKİZOĞLU, Oğuzhan and ORHAN, Nurcan and ARICAN, Nadir and KÜÇÜK, Mutlu and KAYA, Mehmet and ARSLAN, Erol and AHISHALI, Bülent} } @ { tbtkveterinary150719, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, address = {TÜBİTAK}, year = {2014}, volume = {36}, pages = {311 - 318}, doi = {10.3906/sag-1203-43}, title = {The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia}, language = {tr}, key = {cite}, author = {ELMAS, İmdat and KALAYCI, Rivaze and GÜRSES, R.Candan and EKİZOĞLU, Oğuzhan and ORHAN, Nurcan and ARICAN, Nadir and KÜÇÜK, Mutlu and KAYA, Mehmet and ARSLAN, Erol and AHISHALI, Bülent} }
APA KÜÇÜK, M , KAYA, M , ORHAN, N , EKİZOĞLU, O , ARSLAN, E , ARICAN, N , GÜRSES, R , ELMAS, İ , KALAYCI, R , AHISHALI, B . (2014). The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia. Turkish Journal of Veterinary and Animal Sciences, 36 (3), 311-318. Retrieved from http://dergipark.gov.tr/tbtkveterinary/issue/12502/150719
MLA KÜÇÜK, M , KAYA, M , ORHAN, N , EKİZOĞLU, O , ARSLAN, E , ARICAN, N , GÜRSES, R , ELMAS, İ , KALAYCI, R , AHISHALI, B . "The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia". Turkish Journal of Veterinary and Animal Sciences 36 (2014): 311-318 <http://dergipark.gov.tr/tbtkveterinary/issue/12502/150719>
Chicago KÜÇÜK, M , KAYA, M , ORHAN, N , EKİZOĞLU, O , ARSLAN, E , ARICAN, N , GÜRSES, R , ELMAS, İ , KALAYCI, R , AHISHALI, B . "The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia". Turkish Journal of Veterinary and Animal Sciences 36 (2014): 311-318
RIS TY - JOUR T1 - The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia AU - Mutlu KÜÇÜK , Mehmet KAYA , Nurcan ORHAN , Oğuzhan EKİZOĞLU , Erol ARSLAN , Nadir ARICAN , R.Candan GÜRSES , İmdat ELMAS , Rivaze KALAYCI , Bülent AHISHALI Y1 - 2014 PY - 2014 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 311 EP - 318 VL - 36 IS - 3 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2017 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia %A Mutlu KÜÇÜK , Mehmet KAYA , Nurcan ORHAN , Oğuzhan EKİZOĞLU , Erol ARSLAN , Nadir ARICAN , R.Candan GÜRSES , İmdat ELMAS , Rivaze KALAYCI , Bülent AHISHALI %T The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 36 %N 3 %R %U