Yıl 2011, Cilt 35, Sayı 4, Sayfalar 219 - 226 2014-05-18

Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography
Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography

Pradeep KUMAR [1] , S.P. SINGH [2] , K. SHRIKANT [3] , D. MADHUKAR [4]

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Food safety is of great importance to all involved in the food chain. The problem of pesticide residues is one of the concerns for the consumers. In the present study, 254 buffalo liver tissue samples were collected from 4 slaughterhouses and analyzed both qualitatively and quantitatively for the presence of chlorpyrifos residues. The samples were processed by extraction with acetonitrile followed by sonication and liquid-liquid partition. The extracts were cleaned up by performing alumina column chromatography. Chlorpyrifos residues in the samples were detected and quantified by employing high performance liquid chromatography. High performance liquid chromatography (HPLC) was performed by using the isocratic mobile phase, consisting of acetonitrile and water at a ratio of 67:33 with a flow rate of 1 mL/min and a run time of 18 min. The detection wavelength was set at 202 nm with 360 nm as the reference wavelength. Chlorpyrifos residues were detected in 9.05% of the samples. Of these, only 0.78% of the liver samples were found to exceed the Codex maximum residue limit (MRL) for chlorpyrifos.
Food safety is of great importance to all involved in the food chain. The problem of pesticide residues is one of the concerns for the consumers. In the present study, 254 buffalo liver tissue samples were collected from 4 slaughterhouses and analyzed both qualitatively and quantitatively for the presence of chlorpyrifos residues. The samples were processed by extraction with acetonitrile followed by sonication and liquid-liquid partition. The extracts were cleaned up by performing alumina column chromatography. Chlorpyrifos residues in the samples were detected and quantified by employing high performance liquid chromatography. High performance liquid chromatography (HPLC) was performed by using the isocratic mobile phase, consisting of acetonitrile and water at a ratio of 67:33 with a flow rate of 1 mL/min and a run time of 18 min. The detection wavelength was set at 202 nm with 360 nm as the reference wavelength. Chlorpyrifos residues were detected in 9.05% of the samples. Of these, only 0.78% of the liver samples were found to exceed the Codex maximum residue limit (MRL) for chlorpyrifos.
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Yazar: Pradeep KUMAR
E-posta:

Yazar: S.P. SINGH
E-posta:

Yazar: K. SHRIKANT
E-posta:

Yazar: D. MADHUKAR
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Bibtex @ { tbtkveterinary150781, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, address = {TÜBİTAK}, year = {2014}, volume = {35}, pages = {219 - 226}, doi = {}, title = {Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography}, key = {cite}, author = {SINGH, S.P. and KUMAR, Pradeep and SHRIKANT, K. and MADHUKAR, D.} }
APA KUMAR, P , SINGH, S , SHRIKANT, K , MADHUKAR, D . (2014). Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography. Turkish Journal of Veterinary and Animal Sciences, 35 (4), 219-226. Retrieved from http://dergipark.gov.tr/tbtkveterinary/issue/12507/150781
MLA KUMAR, P , SINGH, S , SHRIKANT, K , MADHUKAR, D . "Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography". Turkish Journal of Veterinary and Animal Sciences 35 (2014): 219-226 <http://dergipark.gov.tr/tbtkveterinary/issue/12507/150781>
Chicago KUMAR, P , SINGH, S , SHRIKANT, K , MADHUKAR, D . "Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography". Turkish Journal of Veterinary and Animal Sciences 35 (2014): 219-226
RIS TY - JOUR T1 - Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography AU - Pradeep KUMAR , S.P. SINGH , K. SHRIKANT , D. MADHUKAR Y1 - 2014 PY - 2014 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 219 EP - 226 VL - 35 IS - 4 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2018 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography %A Pradeep KUMAR , S.P. SINGH , K. SHRIKANT , D. MADHUKAR %T Analysis of buffalo liver samples for the presence of chlorpyrifos residues by using high performance liquid chromatography %D 2014 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 35 %N 4 %R %U