Cilt 18, Sayı 1, Sayfalar 33 - 38 2017-04-08

DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)
Beauveria bassiana (BALS.) VULL. İZOLATLARININ Tetranychus urticae KOCH'A (ACARI: TETRANYCHIDAE) KARŞI KONTROL POTANSİYELLERİNİN VE ENZİM AKTİVİTELERİNİN BELİRLENMESİ

Serkan Örtücü [1] , Nurcan ALBAYRAK İSKENDER [2]

204 156

Broad host range of entomopathogenic fungi may have a commercial advantage for biological control industry. In addition, all entomopathogenic fungi rely on a combination of different enzymes to penetrate their hosts and these enzymes play an important role in the host range of fungal isolates. The aim of this study is to determine the potential of three Beauveria bassiana (Bals.) Vull. isolates in control of Tetranychus urticae Koch (Acari: Tetranychidae) and to determine their lipase, chitinase, and protease activities. For this purpose, B. bassiana (PaF04, PaF09 and PaF76) isolates, which was previously demonstrated to have entomopathogenic activity against Coleoptera, Lepidoptera, and Hymenoptera were chosen. T. urticae was used as the test organisms. Three different suspensions (1x106, 1x107 and 1x108 conidia ml-1) were prepared in Tween 80. 2ml suspension of a single dose was sprayed onto down sides of bean leaf discs using hand sprayer. Mortality rates of T. urticae, in terms of LT50 values, were recorded daily for 5 days. Enzyme activities were investigated by petri plate assays and clear zones formed around the colonies indicated enzymatic activities. All tested fungal isolates were pathogenic to the T. urticae causing mortality between 64.2±5.6% and 100% on the 5th day after the application. LT50 values varied between 3.16±0.2 and 3.72±0.5 days. Mortality caused by PaF04 isolate was found to be significantly different (p<0.05). In conclusion, PaF isolates of B. bassiana have been determined to have potential to suppress T. urticae, in addition to their potential in control of Coleoptera, Lepidoptera, and Hymenoptera. The present results showed that the isolates used, especially PaF04, have a broad host range.

Entomopatojen fungusların geniş konukçu çeşitliliği biyolojik mücadele endüstrisi için ticari bir avantaj olabilir. Bununla birlikte, entomopatojen fungusların tümü konukçuya nüfuz etmek için çeşitli enzimlerinin kombinasyonlarını kullanır ve bu enzimler fungal izolatların konukçu çeşitliliğinde önemli bir rol oynar. Bu çalışmanın amacı, üç Beauveria bassiana (Bals.) Vull. izolatının Tetranychus urticae Koch'a (Acari: Tetranychidae) karşı kontrol potansiyellerinin ve lipaz, kitinaz ve proteaz enzim aktivitelerinin belirlenmesidir. Bu amaçla, daha önce Coleoptera, Lepidoptera ve Hymenoptera'ya karşı entomopatojenik aktivite gösteren B. bassiana (PaF04, PaF09 ve PaF76) izolatları seçilmiştir. Test organizması olarak, T. urticae kullanılmıştır. Üç farklı dozda süspansiyon (1x106, 1x107 ve 1x108 konidi ml-1) Tween 80'de hazırlanmıştır. 2ml tek doz spor süspansiyonu el spreyi kullanılarak fasülye yaprak disklerinin alt yüzüne püskürtülmüştür. T. urticae’nın ölüm oranları 5 gün boyunca günlük olarak kaydedilmiştir. Enzim aktiviteleri petri plate yöntemi ile araştırılmış ve koloni çevresinde oluşan açık zonlar, enzimatik aktivitenin varlığını göstermiştir. Test edilen tüm fungus izolatları uygulamadan 5 gün sonra, 64,2±5,6% ve 100% arasında mortaliteye yol açarak T. urticae’ye karşı patojenite göstermiştir. LT50 değerleri 3,16±0,2 ve 3,72±0,5 gün arasında değişmiştir. PaF04 izolatının neden olduğu mortalite önemli derecede farklı bulunmuştur (p<0,05). Sonuçta, B. bassiana PaF izolatlarının Coleoptera, Lepidoptera ve Hymenoptera'yı kontrol potansiyellerinin olduğu kadar T. urticae’yı baskılama potansiyeline de sahip oldukları belirlenmiştir. Mevcut sonuçlar, kullanılan izolatların, özellikle de PaF04'ün geniş bir konukçu aralığına sahip olduğunu göstermiştir.

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Konular Biyoloji
Dergi Bölümü Araştırma Makalesi/Research Article
Yazarlar

Yazar: Serkan Örtücü
E-posta: serkan.ortucu@erzurum.edu.tr
Kurum: ERZURUM TEKNIK UNIV
Ülke: Turkey


Yazar: Nurcan ALBAYRAK İSKENDER
E-posta: nurcaniskender25@hotmail.com
Kurum: ARTVIN CORUH UNIV
Ülke: Turkey


Bibtex @araştırma makalesi { trkjnat285656, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, address = {Trakya Üniversitesi}, year = {2017}, volume = {18}, pages = {33 - 38}, doi = {10.23902/trkjnat.285656}, title = {DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)}, language = {en}, key = {cite}, author = {Örtücü, Serkan and ALBAYRAK İSKENDER, Nurcan} } @araştırma makalesi { trkjnat285656, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, address = {Trakya Üniversitesi}, year = {2017}, volume = {18}, pages = {33 - 38}, doi = {10.23902/trkjnat.285656}, title = {Beauveria bassiana (BALS.) VULL. İZOLATLARININ Tetranychus urticae KOCH'A (ACARI: TETRANYCHIDAE) KARŞI KONTROL POTANSİYELLERİNİN VE ENZİM AKTİVİTELERİNİN BELİRLENMESİ}, language = {tr}, key = {cite}, author = {Örtücü, Serkan and ALBAYRAK İSKENDER, Nurcan} }
APA Örtücü, S , ALBAYRAK İSKENDER, N . (2017). DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE). Trakya University Journal of Natural Sciences, 18 (1), 33-38. DOI: 10.23902/trkjnat.285656
MLA Örtücü, S , ALBAYRAK İSKENDER, N . "DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)". Trakya University Journal of Natural Sciences 18 (2017): 33-38 <http://dergipark.gov.tr/trkjnat/issue/30632/285656>
Chicago Örtücü, S , ALBAYRAK İSKENDER, N . "DETERMINATION OF CONTROL POTENTIALS AND ENZYME ACTIVITIES OF Beauveria bassiana (BALS.) VULL. ISOLATES AGAINST Tetranychus urticae KOCH (ACARI: TETRANYCHIDAE)". Trakya University Journal of Natural Sciences 18 (2017): 33-38
RIS TY - JOUR T1 - Beauveria bassiana (BALS.) VULL. İZOLATLARININ Tetranychus urticae KOCH'A (ACARI: TETRANYCHIDAE) KARŞI KONTROL POTANSİYELLERİNİN VE ENZİM AKTİVİTELERİNİN BELİRLENMESİ AU - Serkan Örtücü , Nurcan ALBAYRAK İSKENDER Y1 - 2017 PY - 2017 N1 - doi: 10.23902/trkjnat.285656 DO - 10.23902/trkjnat.285656 T2 - Trakya University Journal of Natural Sciences JF - Journal JO - JOR SP - 33 EP - 38 VL - 18 IS - 1 SN - 2147-0294-2528-9691 M3 - doi: 10.23902/trkjnat.285656 UR - http://dx.doi.org/10.23902/trkjnat.285656 Y2 - 2017 ER -
EndNote %0 Trakya University Journal of Natural Sciences Beauveria bassiana (BALS.) VULL. İZOLATLARININ Tetranychus urticae KOCH'A (ACARI: TETRANYCHIDAE) KARŞI KONTROL POTANSİYELLERİNİN VE ENZİM AKTİVİTELERİNİN BELİRLENMESİ %A Serkan Örtücü , Nurcan ALBAYRAK İSKENDER %T Beauveria bassiana (BALS.) VULL. İZOLATLARININ Tetranychus urticae KOCH'A (ACARI: TETRANYCHIDAE) KARŞI KONTROL POTANSİYELLERİNİN VE ENZİM AKTİVİTELERİNİN BELİRLENMESİ %D 2017 %J Trakya University Journal of Natural Sciences %P 2147-0294-2528-9691 %V 18 %N 1 %R doi: 10.23902/trkjnat.285656 %U 10.23902/trkjnat.285656