Cilt 18, Sayı 1, Sayfalar 49 - 54 2017-05-22

3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ
SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL ACTIVITIES OF 3-(2-HYDROXYETHYL)-1-(3-AMINOPROPYL)-1H-IMIDAZOLE-3-IUM BROMIDE

gühergül uluçam [1]

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Bir N-heterohalka karben tuzu olarak, 3-(2-hidroksietil)-1-(3-aminopropil)-1H-imidazol-3-ium bromid, 3-(1H-imidazol-1-il)propan-1-amin ile 2-bromoetanol’ün reaksiyonu sonucunda sentezlenmiştir. Tuz, N-heterohalka karbenlerin en kararlı gruplarından biri olan imidazol tuzu olarak da isimlendirilebilir. Ürünün moleküler yapısı FTIR, 1HNMR, 13C NMR, GS-MS, elemental analiz yöntemleri ile aydınlatılmıştır. Ayrıca, antimikrobiyal, antifungal ve antikanser aktiviteleri incelenmiştir. Probit analizi yapılarak, HeLa ve HepG2 kanser hücre serileri üzerinde 24 saatlik uygulama sonrası IC50 değerlerinin sırasıyla 155,955µM ve 76,769µM olduğu saptanmıştır. Sentezlenen maddenin sağlıklı fare embriyonik fibroblast (MEF) hücreleri üzerinde zararlı bir etkisine rastlanmamıştır. Escherichia coli (Migula, 1895) O157:H7, Listeria monocytogenes (Pirie, 1940) ATCC 19115 bakterileri ve Candida albicans (Berhout, 1923) ATCC 10231 mayası üzerinde ise 32µM’lık konsantrasyonda kayda değer antimikrobiyal ve antifungal aktivitesinin de olduğu belirlenmiştir.

3-(2-hydroxyethyl)-1-(3-aminopropyl)-1H-imidazole-3-ium bromide as an N-heterocyclic carbene salt was synthesized by the reaction of 3-(1H-imidazole-1-yl )propane -1-amine with 2-bromoethanol. The salt can also be named as imidazole salt which is one of the most stable group of N-heterocyclic carbenes.  The molecular structure of the product was elucidated by FTIR, 1H NMR, 13C NMR, GS-MS and elemental analysis. The antimicrobial, antifungal and anticancer activities of the imidazole salt were also examined. The probit analysis revealed that IC50 values on HeLa and HepG2 cell lines were 155.955μM and 76.769μM, respectively, at the end of 24 hours treatment. It also was determined that the product has no harmful effect on healthy mouse embryonic fibroblasts (MEF) cells. Significant antimicrobial and antifungal activities of the salt on Escherichia coli (Migula, 1895) O157:H7 and Listeria monocytogenes (Pirie, 1940) ATCC 19115 bacteria and the yeast Candida albicans (Berhout, 1923) ATCC 10231 were determined at a concentration value of 32μM.

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Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makalesi/Research Article
Yazarlar

Yazar: gühergül uluçam
E-posta: gulergul@trakya.edu.tr
Kurum: Trakya Üniversitesi, Kimya Bölümü, Anorganik Kimya Anabilim Dalı, Edirne
Ülke: Turkey


Bibtex @araştırma makalesi { trkjnat291076, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, address = {Trakya Üniversitesi}, year = {2017}, volume = {18}, pages = {49 - 54}, doi = {10.23902/trkjnat.291076}, title = {3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ}, language = {tr}, key = {cite}, author = {uluçam, gühergül} } @araştırma makalesi { trkjnat291076, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, address = {Trakya Üniversitesi}, year = {2017}, volume = {18}, pages = {49 - 54}, doi = {10.23902/trkjnat.291076}, title = {SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL ACTIVITIES OF 3-(2-HYDROXYETHYL)-1-(3-AMINOPROPYL)-1H-IMIDAZOLE-3-IUM BROMIDE}, language = {en}, key = {cite}, author = {uluçam, gühergül} }
APA uluçam, g . (2017). 3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ. Trakya University Journal of Natural Sciences, 18 (1), 49-54. DOI: 10.23902/trkjnat.291076
MLA uluçam, g . "3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ". Trakya University Journal of Natural Sciences 18 (2017): 49-54 <http://dergipark.gov.tr/trkjnat/issue/30632/291076>
Chicago uluçam, g . "3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ". Trakya University Journal of Natural Sciences 18 (2017): 49-54
RIS TY - JOUR T1 - 3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ AU - gühergül uluçam Y1 - 2017 PY - 2017 N1 - doi: 10.23902/trkjnat.291076 DO - 10.23902/trkjnat.291076 T2 - Trakya University Journal of Natural Sciences JF - Journal JO - JOR SP - 49 EP - 54 VL - 18 IS - 1 SN - 2147-0294-2528-9691 M3 - doi: 10.23902/trkjnat.291076 UR - http://dx.doi.org/10.23902/trkjnat.291076 Y2 - 2017 ER -
EndNote %0 Trakya University Journal of Natural Sciences 3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ %A gühergül uluçam %T 3-(2-HİDROKSİETİL)-1-(3-AMİNOPROPİL)-1H-İMİDAZOL-3-İUM BROMİD SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİ %D 2017 %J Trakya University Journal of Natural Sciences %P 2147-0294-2528-9691 %V 18 %N 1 %R doi: 10.23902/trkjnat.291076 %U 10.23902/trkjnat.291076