Year 2019, Volume 6, Issue 1, Pages 97 - 105 2019-01-21

Kombine Tuz ve Nikel Stresinin Limon Otu (Melissa officinalis)'nun ROS Üretimi ve Antioksidan Enzim Aktiviteleri Üzerine Etkisi
Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis)

Hülya TORUN [1]

20 83

Bitkilerin strese alışması karmaşık bir süreçtir ve bu karmaşıklık iki veya daha fazla abiyotik strese aynı anda maruz kalındığında artar. Tuzluluk ve aşırı nikel birikimi büyüme ve gelişimi sınırlandıran abiyotik ajanlardır. Bu çalışmanın amacı, Melissa officinalis (limon otu) bitkisinde tuzluluk, nikel ve bunların kombinasyonlarına karşı antioksidan savunma sistem cevaplarını belirlemektir. Bu stres koşulları altında, büyüme, yaprak ozmotik potansiyeli, klorofil floresansı, nisbi su içeriği (RWC), lipid peroksidasyonu, H2O2 ve prolin içeriği ve antioksidan savunma enzimleri belirlenmiştir. Büyüme ve yaprak osmotik potansiyeli tuz ve nikel kombinasyonları nedeniyle olumsuz etkilenmiştir. Her üç stres uygulaması, limon otunun RWC ve klorofil floresansında önemli değişiklik göstermemiştir. Birleşik stres altındaki H2O2 içeriği ve lipid peroksidasyonu, tuz ve nikelin tekli uygulamalarından daha yüksektir. Askorbat peroksidaz (APX) ve glutatyon redüktaz (GR)’ın toplam aktiviteleri tuz, nikel ve kombinasyonları ile artmıştır. Dahası, süperoksit dismutaz (SOD), peroksidaz (POX) ve katalaz (CAT) aktivitelerindeki düşüş, birleşik stres altında yüksek lipid peroksidasyonu ve H2O2 içeriğinin artması ile sonuçlanmıştır. Bildiğimiz en iyi şekilde, bu çalışma tuzluluk ve nikel stresleri kombinasyonları altında limon otunun antioksidan savunma enzim aktivitelerinin yürütüldüğü ilk çalışmadır.

Plants acclimation to stress is a complex process and this complexity increase with simultaneous subjection to two or more abiotic stress. Salinity and excess accumulation of nickel are growth and development restricted abiotic stress agents. The aim of the present study was to determine the antioxidant defense system responses of Melissa officinalis (lemon balm) to salinity, nickel and their combinations. Growth, leaf osmotic potential, chlorophyll fluorescence, relative water content (RWC), lipid peroxidation, H2O2 and proline content and antioxidant enzyme activities were determined under these stress conditions. Growth and leaf osmotic potential negatively affected due to the combined effect of salt and nickel. All three stress treatments did not show any significant change on RWC and chlorophyll fluorescence in lemon balm. H2O2 content and lipid peroxidation of combined stress had greater than salt and nickel alone. Total activities of ascorbate peroxidase (APX) and glutathione reductase (GR) were increased with salt, nickel and their combinations. Moreover, the reduction in superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) activities resulted in higher lipid peroxidation and H2O2 content under the combined stress. To the best of our knowledge, this is the first study conducted on the activities of antioxidant defense enzymes of lemon balm under salinity and nickel combinations.

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Primary Language en
Subjects Science
Journal Section Research Articles
Authors

Author: Hülya TORUN

Bibtex @research article { turkjans515361, journal = {Türk Tarım ve Doğa Bilimleri Dergisi}, issn = {2148-3647}, address = {Mevlüt AKÇURA}, year = {2019}, volume = {6}, pages = {97 - 105}, doi = {10.30910/turkjans.515361}, title = {Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis)}, key = {cite}, author = {TORUN, Hülya} }
APA TORUN, H . (2019). Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis). Türk Tarım ve Doğa Bilimleri Dergisi, 6 (1), 97-105. DOI: 10.30910/turkjans.515361
MLA TORUN, H . "Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis)". Türk Tarım ve Doğa Bilimleri Dergisi 6 (2019): 97-105 <http://dergipark.gov.tr/turkjans/issue/42713/515361>
Chicago TORUN, H . "Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis)". Türk Tarım ve Doğa Bilimleri Dergisi 6 (2019): 97-105
RIS TY - JOUR T1 - Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis) AU - Hülya TORUN Y1 - 2019 PY - 2019 N1 - doi: 10.30910/turkjans.515361 DO - 10.30910/turkjans.515361 T2 - Türk Tarım ve Doğa Bilimleri Dergisi JF - Journal JO - JOR SP - 97 EP - 105 VL - 6 IS - 1 SN - 2148-3647- M3 - doi: 10.30910/turkjans.515361 UR - https://doi.org/10.30910/turkjans.515361 Y2 - 2019 ER -
EndNote %0 Turkish Journal of Agricultural and Natural Sciences Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis) %A Hülya TORUN %T Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis) %D 2019 %J Türk Tarım ve Doğa Bilimleri Dergisi %P 2148-3647- %V 6 %N 1 %R doi: 10.30910/turkjans.515361 %U 10.30910/turkjans.515361
ISNAD TORUN, Hülya . "Combined Salt and Nickel Stress Impact on ROS Generation and Antioxidant Enzymes Activities of Lemon Balm (Melissa officinalis)". Türk Tarım ve Doğa Bilimleri Dergisi 6 / 1 (January 2019): 97-105. https://doi.org/10.30910/turkjans.515361