Cilt 17, Sayı 3, Sayfalar 283 - 294 2017-09-01

Automatic Cells Counting in Natt-Herrick Stained Fish Blood
Automatic Cells Counting in Natt-Herrick Stained Fish Blood

M. Ozan İncetaş [1] , Erdinç VESKE [2] , Nesrin EMRE [3] , Recep DEMİRCİ [4]

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Hematolojik değerlerin kontrolü balıkların sağlık durumları hakkında önemli bilgiler verdiğinden, bu kontrol balık yetiştiriciliğinde oldukça önemlidir. Hematolojik değerlerin kontrolünde en sık kullanılan yöntemlerden biri de Natt-Herrick solüsyonudur. Natt-Herrick solüsyonu yardımıyla boyanan kan örnekleri mikroskop ile incelenmekte ve hücre sayımı yapılmaktadır. Ancak bu sayım hem yorucu hem de zaman alıcıdır. Bu çalışmada kan örneklerine ait görüntüler üzerinden otomatik olarak hücre sayımı yapabilecek bir teknik sunulmuştur. Geliştirilen yöntemin değerlendirilmesinde Natt-Herrick solüsyonu ile boyanmış Gökkuşağı Alabalığı ve Çipura balıklarına ait örnekler kullanılmıştır. Bu örneklere ait 90 adet görüntüdeki kan hücrelerinin otomatik belirlenmesi sonuçları ile kullanıcılar tarafından belirlenen sonuçlarla karşılaştırılmıştır. Karşılaştırma sonucunda ortalama olarak 0,96’nın üzerinde f-skor değeri elde edilmiştir.

Monitoring of hematological values which provide important information about the health status of fish is considerably important in aquaculture. One of the most commonly used methods for detecting the hematological values in fish blood is the usage of Natt-Herrick solution.   Basically, in this approach, Natt-Herrick stained blood samples are examined with a microscope and the cells are counted. Nevertheless, the counting process is both tough and time-consuming. In this study, a technique in which cell counting in blood samples images is automatically performed has been presented. Natt-Herrick stained blood samples of Oncorhynchus mykiss and Sparus aurata were used for evaluation of the developed scheme. The outputs generated by automatic blood cells detection algorithm in 90 images were compared with results which were obtained by means of user’s intervention.  Consequently, an average f-score over 0.96 was achieved.


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Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: M. Ozan İncetaş
E-posta: ozanincetas@beun.edu.tr
Kurum: Bülent Ecevit Üniversitesi, Mühendislik Fakültesi
Ülke: Turkey


Yazar: Erdinç VESKE
E-posta: eveske@tagem.gov.tr
Kurum: Gıda Tarım ve Hayvancılık Bakanlığı, Tarımsal Araştırmalar Genel Müdürlüğü
Ülke: Turkey


Yazar: Nesrin EMRE
E-posta: nemre70@gmail.com
Kurum: Akdeniz Su Ürünleri Araştırma Enstitüsü
Ülke: Turkey


Yazar: Recep DEMİRCİ
E-posta: rdemirci@gazi.edu.tr
Kurum: Gazi Üniversitesi, Teknoloji Fakültesi
Ülke: Turkey


Bibtex @araştırma makalesi { yunusae306490, journal = {Yunus Araştırma Bülteni}, issn = {1303-4456}, address = {Su Ürünleri Merkez Araştırma Enstitüsü}, year = {2017}, volume = {17}, pages = {283 - 294}, doi = {10.17693/yunusae.v17i30729.306490}, title = {Automatic Cells Counting in Natt-Herrick Stained Fish Blood}, language = {tr}, key = {cite}, author = {DEMİRCİ, Recep and EMRE, Nesrin and İncetaş, M. Ozan and VESKE, Erdinç} } @araştırma makalesi { yunusae306490, journal = {Yunus Araştırma Bülteni}, issn = {1303-4456}, address = {Su Ürünleri Merkez Araştırma Enstitüsü}, year = {2017}, volume = {17}, pages = {283 - 294}, doi = {10.17693/yunusae.v17i30729.306490}, title = {Automatic Cells Counting in Natt-Herrick Stained Fish Blood}, language = {en}, key = {cite}, author = {DEMİRCİ, Recep and EMRE, Nesrin and İncetaş, M. Ozan and VESKE, Erdinç} }
APA İncetaş, M , VESKE, E , EMRE, N , DEMİRCİ, R . (2017). Automatic Cells Counting in Natt-Herrick Stained Fish Blood. Yunus Araştırma Bülteni, 17 (3), 283-294. DOI: 10.17693/yunusae.v17i30729.306490
MLA İncetaş, M , VESKE, E , EMRE, N , DEMİRCİ, R . "Automatic Cells Counting in Natt-Herrick Stained Fish Blood". Yunus Araştırma Bülteni 17 (2017): 283-294 <http://dergipark.gov.tr/yunusae/issue/30729/306490>
Chicago İncetaş, M , VESKE, E , EMRE, N , DEMİRCİ, R . "Automatic Cells Counting in Natt-Herrick Stained Fish Blood". Yunus Araştırma Bülteni 17 (2017): 283-294
RIS TY - JOUR T1 - Automatic Cells Counting in Natt-Herrick Stained Fish Blood AU - M. Ozan İncetaş , Erdinç VESKE , Nesrin EMRE , Recep DEMİRCİ Y1 - 2017 PY - 2017 N1 - doi: 10.17693/yunusae.v17i30729.306490 DO - 10.17693/yunusae.v17i30729.306490 T2 - Yunus Araştırma Bülteni JF - Journal JO - JOR SP - 283 EP - 294 VL - 17 IS - 3 SN - 1303-4456-2149-1828 M3 - doi: 10.17693/yunusae.v17i30729.306490 UR - http://dx.doi.org/10.17693/yunusae.v17i30729.306490 Y2 - 2017 ER -
EndNote %0 Yunus Araştırma Bülteni Automatic Cells Counting in Natt-Herrick Stained Fish Blood %A M. Ozan İncetaş , Erdinç VESKE , Nesrin EMRE , Recep DEMİRCİ %T Automatic Cells Counting in Natt-Herrick Stained Fish Blood %D 2017 %J Yunus Araştırma Bülteni %P 1303-4456-2149-1828 %V 17 %N 3 %R doi: 10.17693/yunusae.v17i30729.306490 %U 10.17693/yunusae.v17i30729.306490