Cilt 27, Sayı 3, Sayfalar 318 - 325 2017-09-29

The Effects of Photoperiod, Jasmonic Acid and Activated Charcoal on in vitro microtuberization in Potatoes (Solanum tuberosum L.)
Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Fotoperiyot, Jasmonik Asit ve Aktif Kömürün Etkileri

Seray KENAR [1] , Gökçen BAYSAL FURTANA [2] , Şeküre Şebnem ELLİALTIOĞLU [3] , Rukiye TIPIRDAMAZ [4]

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In this study, effects of added agar, jasmonic acid (JA), activate charcoal (AC) with photoperiod regime on in vitro microtuber formation in Solanum tuberosum cv. Marfona. Study has actualized as 4 main steps; shoot formation of the tubers of S. tuberosum, in vitro shoot-tip culture, micropropagation and microtuberization. The effects of JA (0.0-0.2 mgL-1), agar (0.5% or 0.7%), AC (0 and 0.2%) microtuberization were investigated. The highest microtuber yield (137%) and the highest microtuber average (183 mg/tuber) were obtained at the double-layer Murashige and Skoog (MS) medium which contains 0.2% AC, 0.5% agar under darkness conditions. It has been determined that positive effect of AC on microtuber formation is higher than JA. AC was found much more effective in the dark.

Bu çalışmada, ekonomik değeri olan Solanum tuberosum L. cv. Marfona’da in vitro mikrotuber oluşumu üzerinde fotoperiyot ile ortama ilave edilen Jasmonik asit (JA), aktif kömür (AC) ve agarın etkileri araştırılmıştır. Bu amaçla çalışma 4 aşamada gerçekleştirilmiştir: Tuberlerden sürgün oluşumu, in vitro sürgün ucu kültürü, mikroçoğaltım ve mikrotuberizasyon. Sürgün ucu kültürü ile in vitro çoğaltılan bitkilerden alınan tek boğum eksplantları JA (0.0-0.2 mg L-1), agar (%0.5, %0.7) ve AC (0, %0.2) içeren çift fazlı Murashige and Skoog (MS) ortamlarında 2 farklı fotoperiyotta kısa gün (8/16 saat aydınlık/karanlık veya sürekli karanlık) kültüre alınmıştır. En yüksek mikrotuber verimi (%137) ve en yüksek ortalama mikrotuber ağırlığı (183 mg); % 0.2 AC, %0.5 agar içeren çift fazlı MS ortamında ve karanlıkta elde edilmiştir. AC’nin mikrotuber oluşumu üzerindeki olumlu etkisinin JA’den daha fazla olduğu ve AC’nin karanlıkta daha etkili olduğu belirlenmiştir.

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Konular Yaşam Bilimleri ,Mühendislik ve Temel Bilimler
Yayımlanma Tarihi Eylül
Dergi Bölümü Makaleler
Yazarlar

Yazar: Seray KENAR
E-posta: tuz@hacattepe.edu.tr
Kurum: Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Beytepe, Ankara
Ülke: Turkey


Yazar: Gökçen BAYSAL FURTANA
E-posta: gokcenbaysal@gmail.com
Kurum: Gazi Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Teknikokullar, Ankara
Ülke: Turkey


Yazar: Şeküre Şebnem ELLİALTIOĞLU
E-posta: sebnemellialti@gmail.com
Kurum: Ankara Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Dışkapı, Ankara
Ülke: Turkey


Yazar: Rukiye TIPIRDAMAZ
E-posta: tuz@hacettepe.edu.tr
Kurum: Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Beytepe, Ankara
Ülke: Turkey


Bibtex @araştırma makalesi { yyutbd285085, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, address = {Yüzüncü Yıl Üniversitesi}, year = {2017}, volume = {27}, pages = {318 - 325}, doi = {}, title = {The Effects of Photoperiod, Jasmonic Acid and Activated Charcoal on in vitro microtuberization in Potatoes (Solanum tuberosum L.)}, language = {en}, key = {cite}, author = {ELLİALTIOĞLU, Şeküre Şebnem and KENAR, Seray and TIPIRDAMAZ, Rukiye and BAYSAL FURTANA, Gökçen} } @araştırma makalesi { yyutbd285085, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, address = {Yüzüncü Yıl Üniversitesi}, year = {2017}, volume = {27}, pages = {318 - 325}, doi = {}, title = {Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Fotoperiyot, Jasmonik Asit ve Aktif Kömürün Etkileri}, language = {tr}, key = {cite}, author = {ELLİALTIOĞLU, Şeküre Şebnem and KENAR, Seray and TIPIRDAMAZ, Rukiye and BAYSAL FURTANA, Gökçen} }
APA KENAR, S , BAYSAL FURTANA, G , ELLİALTIOĞLU, Ş , TIPIRDAMAZ, R . (2017). The Effects of Photoperiod, Jasmonic Acid and Activated Charcoal on in vitro microtuberization in Potatoes (Solanum tuberosum L.). Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 27 (3), 318-325. Retrieved from http://dergipark.gov.tr/yyutbd/issue/31486/285085
MLA KENAR, S , BAYSAL FURTANA, G , ELLİALTIOĞLU, Ş , TIPIRDAMAZ, R . "The Effects of Photoperiod, Jasmonic Acid and Activated Charcoal on in vitro microtuberization in Potatoes (Solanum tuberosum L.)". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 27 (2017): 318-325 <http://dergipark.gov.tr/yyutbd/issue/31486/285085>
Chicago KENAR, S , BAYSAL FURTANA, G , ELLİALTIOĞLU, Ş , TIPIRDAMAZ, R . "The Effects of Photoperiod, Jasmonic Acid and Activated Charcoal on in vitro microtuberization in Potatoes (Solanum tuberosum L.)". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 27 (2017): 318-325
RIS TY - JOUR T1 - Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Fotoperiyot, Jasmonik Asit ve Aktif Kömürün Etkileri AU - Seray KENAR , Gökçen BAYSAL FURTANA , Şeküre Şebnem ELLİALTIOĞLU , Rukiye TIPIRDAMAZ Y1 - 2017 PY - 2017 N1 - DO - T2 - Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 318 EP - 325 VL - 27 IS - 3 SN - 1308-7576-1308-7584 M3 - UR - Y2 - 2017 ER -
EndNote %0 Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Fotoperiyot, Jasmonik Asit ve Aktif Kömürün Etkileri %A Seray KENAR , Gökçen BAYSAL FURTANA , Şeküre Şebnem ELLİALTIOĞLU , Rukiye TIPIRDAMAZ %T Patateste (Solanum tuberosum L.) in vitro Mikrotuberizasyon Üzerine Fotoperiyot, Jasmonik Asit ve Aktif Kömürün Etkileri %D 2017 %J Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi %P 1308-7576-1308-7584 %V 27 %N 3 %R %U