Yıl 2018, Cilt 28, Sayı 1, Sayfalar 1 - 9 2018-03-30

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome
Fasulye Genomunda 2,4-Diklorofenoksiasetik Asit Genotoksisitesine Karşı Β-Östradiolün Hafifletici Rolü

Mahmut Sinan TAŞPINAR [1] , Burcu SIĞMAZ [2] , Murat AYDIN [3] , Esra ARSLAN [4] , Güleray AĞAR [5]

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2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the several herbicides that widely used to prevent development of Taraxacum officinale and broadleaf weeds on cereal crops culture. However, it is known that 2,4-D can cause genetic damage to plants at low concentrations as well as produce observable physiological effects. There is no report investigating the effect of mammalian hormones in crops against the applied 2,4-D. Therefore, the present study was aimed at investigating levels of DNA damage, changes in DNA methylation and DNA stability in common bean (Phaseolus vulgaris) exposed to 2,4-D and determine whether β-estradiol has any effect. RAPDs (Randomly Amplified Polymorphic DNA) and CRED-RAs (Coupled Restriction Enzyme Digestion-Random Amplification) techniques were used to define the DNA damage levels and changes in the pattern of DNA methylation.  The obtained data demonstrated that 2,4-D led to an increase in RAPDs profile changes (DNA damage), and a reduction in genomic template stability (GTS). The effects caused by 2,4-D were decreased after application with different concentrations of β-estradiol. The results of this study clearly show that β-estradiol could be used function effectively to prevented from the genetic and epigenetic changes caused by 2,4-D herbicide in common bean.

2,4-Diklorofenoksiasetik asit (2,4-D) tahıl ekin kültüründe Taraxacum officinale ve geniş yapraklı yabancı otların gelişmesini önlemek için yaygın olarak kullanılan herbisitlerden biridir. Ancak, 2,4-D'nin, düşük konsantrasyonlarda bitkilerde genetik hasarın yanı sıra gözlemlenebilir fizyolojik etkilere neden olduğu bilinmektedir. Uygulanan 2,4-D'ye karşı bitkilerde memeli hormonlarının etkisini araştıran herhangi bir rapor bulunmamaktadır. Bu nedenle, bu çalışma 2,4-D'ye maruz bırakılan fasulye (Phaseolus vulgaris)'deki DNA hasarı, DNA metilasyonu ve DNA stabilitesindeki değişimleri araştırmayı ve β-östradiolün herhangi bir etkisinin olup olmadığını belirlemeyi amaçlamıştır. DNA hasar düzeylerini ve DNA metilasyon modelindeki değişiklikleri tanımlamak için RAPD (Rastgele Çoğaltılan Polimorfik DNA) ve CRED-RA (Çift Restriksiyon Enzim Kesimi-Rastgele Çoğaltımı) teknikleri kullanılmıştır. Elde edilen veriler, 2,4-D'nin RAPD profil değişikliklerinde (DNA hasarında) artışa ve genomik kararlılık stabilitesinde (GTS) ise azalışa neden olduğunu göstermiştir. 2,4-D'nin neden olduğu etkiler, farklı β-östradiol konsantrasyonları uygulandıktan sonra azalmıştır. Bu çalışmanın sonuçları, fasulyelerde 2,4-D herbisitin neden olduğu genetik ve epigenetik değişikliklerin engellenmesi için β-östradiolün etkin bir şekilde kullanılabileceğini açıkça göstermektedir.

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Birincil Dil en
Konular Yaşam Bilimleri, Mühendislik ve Temel Bilimler
Dergi Bölümü Makaleler
Yazarlar

Yazar: Mahmut Sinan TAŞPINAR

Yazar: Burcu SIĞMAZ

Yazar: Murat AYDIN (Sorumlu Yazar)
Ülke: Turkey


Yazar: Esra ARSLAN

Yazar: Güleray AĞAR

Bibtex @araştırma makalesi { yyutbd305274, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, eissn = {1308-7584}, address = {Yüzüncü Yıl Üniversitesi}, year = {2018}, volume = {28}, pages = {1 - 9}, doi = {10.29133/yyutbd.305274}, title = {Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome}, key = {cite}, author = {AYDIN, Murat and TAŞPINAR, Mahmut Sinan and AĞAR, Güleray and ARSLAN, Esra and SIĞMAZ, Burcu} }
APA TAŞPINAR, M , SIĞMAZ, B , AYDIN, M , ARSLAN, E , AĞAR, G . (2018). Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 28 (1), 1-9. DOI: 10.29133/yyutbd.305274
MLA TAŞPINAR, M , SIĞMAZ, B , AYDIN, M , ARSLAN, E , AĞAR, G . "Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 (2018): 1-9 <http://dergipark.gov.tr/yyutbd/issue/36698/305274>
Chicago TAŞPINAR, M , SIĞMAZ, B , AYDIN, M , ARSLAN, E , AĞAR, G . "Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 (2018): 1-9
RIS TY - JOUR T1 - Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome AU - Mahmut Sinan TAŞPINAR , Burcu SIĞMAZ , Murat AYDIN , Esra ARSLAN , Güleray AĞAR Y1 - 2018 PY - 2018 N1 - doi: 10.29133/yyutbd.305274 DO - 10.29133/yyutbd.305274 T2 - Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 1 EP - 9 VL - 28 IS - 1 SN - 1308-7576-1308-7584 M3 - doi: 10.29133/yyutbd.305274 UR - http://dx.doi.org/10.29133/yyutbd.305274 Y2 - 2018 ER -
EndNote %0 Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome %A Mahmut Sinan TAŞPINAR , Burcu SIĞMAZ , Murat AYDIN , Esra ARSLAN , Güleray AĞAR %T Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome %D 2018 %J Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi %P 1308-7576-1308-7584 %V 28 %N 1 %R doi: 10.29133/yyutbd.305274 %U 10.29133/yyutbd.305274
ISNAD TAŞPINAR, Mahmut Sinan , SIĞMAZ, Burcu , AYDIN, Murat , ARSLAN, Esra , AĞAR, Güleray . "Fasulye Genomunda 2,4-Diklorofenoksiasetik Asit Genotoksisitesine Karşı Β-Östradiolün Hafifletici Rolü". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 / 1 (Mart 2018): 1-9. http://dx.doi.org/10.29133/yyutbd.305274