Yıl 2018, Cilt 28, Sayı 1, Sayfalar 126 - 134 2018-03-30

Entomolojide DNA Barkodlama Tekniğinin Kullanımı
The Use of DNA Barcoding in Entomology

Gökhan YATKIN [1] , Nurper GÜZ [2]

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DNA barkodlama, herhangi bir organizmaya ait DNA’nın PCR teknikleri ile çoğaltılacak büyüklükteki kısa ve standart bir fragmanının dizilenmesi ile tanılanmasını sağlayan taksonomik bir yöntem olarak ifade edilebilir. Bu amaçla böceklerle yapılan çalışmalarda ‘DNA barkodu’ olarak bilinen mitokondriyal sitokrom c oksidaz alt ünite I (COI) geninin yaklaşık 600-700 baz çiftine (bç) ait kısmı kullanılmaktadır. Bu kısa standart DNA sekansı türlerin tanılanmasını kolaylaştırmasının yanı sıra yeni türlerin keşfi için de oldukça iyi bir araçtır. Ayrıca DNA barkodlama türlerin sınırlarını belirleyerek kriptik türlerin ayrımına katkı sağlar. Bu derlemede DNA barkodlama teriminin tarihsel ve kavramsal olarak ortaya çıkışı, DNA barkodu olarak kullanılan gen bölgeleri ve böcekler için ideal tür tanımlama markörü olan COI gen bölgesi hakkında bilgi verilmiştir. Son olarak DNA barkodlamanın avantajları ve kısıtlamaları irdelenerek entomolojide DNA barkodlama çalışmalarından örnekler sunulmuştur. 

DNA barcoding is a taxonomic method that uses a short and standard genomic DNA fragment to discriminate species. An approximately 600-700 base pair (bp) region of the mitochondrial cytochrome c oxidase subunit I (COI) gene referred as ‘DNA barcode’ is used in insect barcoding studies. This short standart DNA sequence is not only a tool for simplifying species identification but also is used for discovery of new species. Furthermore DNA barcoding enables the detection of cryptic species via delimiting species boundaries. Here we reviewed the historical and conceptual perspective of DNA barcoding term, the gene regions used as DNA barcodes and COI gene which is ideal species-identification marker in insects. Furthermore we discussed the advantages and limitations of DNA barcoding and presented DNA barcoding studies in entomology.

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Birincil Dil tr
Konular Yaşam Bilimleri
Dergi Bölümü Makaleler
Yazarlar

Yazar: Gökhan YATKIN
E-posta: gytkn@hotmail.com
Kurum: Ankara Zirai Mücadele Merkez Araştırma Enstitüsü
Ülke: Turkey


Yazar: Nurper GÜZ (Sorumlu Yazar)
E-posta: nurperguz@agri.ankara.edu.tr
Kurum: Ankara Üniversitesi Ziraat Fakültesi Bitki Koruma Bölümü Moleküler Entomoloji Laboratuvarı
Ülke: Turkey


Bibtex @derleme { yyutbd350146, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, address = {Yüzüncü Yıl Üniversitesi}, year = {2018}, volume = {28}, pages = {126 - 134}, doi = {10.29133/yyutbd.350146}, title = {Entomolojide DNA Barkodlama Tekniğinin Kullanımı}, key = {cite}, author = {GÜZ, Nurper and YATKIN, Gökhan} }
APA YATKIN, G , GÜZ, N . (2018). Entomolojide DNA Barkodlama Tekniğinin Kullanımı. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 28 (1), 126-134. DOI: 10.29133/yyutbd.350146
MLA YATKIN, G , GÜZ, N . "Entomolojide DNA Barkodlama Tekniğinin Kullanımı". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 (2018): 126-134 <http://dergipark.gov.tr/yyutbd/issue/36698/350146>
Chicago YATKIN, G , GÜZ, N . "Entomolojide DNA Barkodlama Tekniğinin Kullanımı". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 (2018): 126-134
RIS TY - JOUR T1 - Entomolojide DNA Barkodlama Tekniğinin Kullanımı AU - Gökhan YATKIN , Nurper GÜZ Y1 - 2018 PY - 2018 N1 - doi: 10.29133/yyutbd.350146 DO - 10.29133/yyutbd.350146 T2 - Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 126 EP - 134 VL - 28 IS - 1 SN - 1308-7576-1308-7584 M3 - doi: 10.29133/yyutbd.350146 UR - http://dx.doi.org/10.29133/yyutbd.350146 Y2 - 2018 ER -
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