Yıl 2018, Cilt 28, Sayı 2, Sayfalar 186 - 191 2018-06-29

Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil
Juniperus oxycedrus L. subsp. oxycedrus Yapraklarındaki Uçucu Yağın Kimyasal Yapısı ve Antibakteriyel Özelliklerinin Belirlenmesi

Neşe OKUT [1] , Bünyamin YILDIRIM [2] , Kamil EKİCİ [3] , Ömer TERZİOĞLU [4] , Fevzi ÖZGÖKÇE [5]

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The constituents of leaf essential oil of Juniperus oxycedrus L. subsp. oxycedrus (JOO) from wild flora of Gevas-Van in Turkey (Mount Artos) were studied using Gas Chromatography-Mass Spectrometry (GC-MS) and their antibacterial activities were assessed. A total of 18 compounds representing 99.98% of leaves oil were identified. The oils of the plant are all dominated by monoterpenes. The main compounds of essential oil in leaves were Limonene (45.77%), α-Pinene (23.94%), β-Phellandrene (10.83%), β-Pinene (5.68%), o-Cymene (3.30%), respectively. The essential oils were tested against Staphylacoccus aureus, Bacillus subtilis, Pseudomanas aeruginosa, Enterecoccus faecalis, Salmonella typhimurium, and Escherichia coli strains using the disc diffusion method. The diameter of the inhibition zones formed for bacteria were measured. The essential oils of JOO found to be active against all of the tested microorganisms and showed the susceptible inhibition zones. However, they were not as much as effective against bacterial strains when compared to ampicillin and ofloxacin. The extracts of  JOO showed most significant antibacterial activity against Escherichia coli with inhibition zone diameter of 15 mm. The lowest inhibition zone diameter was Staphylococcus aureus with 11 mm. 

Türkiye’de Van ilinin Gevaş ilçesindeki Artos Dağı doğal ortamından toplanan Juniperus oxycedrus L. subsp. oxycedrus (JOO) (Ardıç, Dikenli ardıç) yapraklarının uçucu yağ bileşenleri Gaz Kromatografisi-Kütle Spektrometresi (GC-MS) ile araştırılmış ve sonrasında da antibakteriyel aktivitesi belirlenmiştir. Yapraklardaki uçucu yağın % 99.98’ini temsil edecek şekilde toplam 18 bileşen tespit edilmiştir. J. oxycedrus L. subsp. oxycedrus (JOO) uçucu yağının çoğunluğuu monoterpenler oluşturmuştur. Yapraklarda bulunan önemli bileşenler sırasıyla Limonen (45.77%), α-Pinen (23.94%), β-Phellandren (10.83%), β-Pinen (5.68%), o-Cymen (3.30%)’dir. Disk diffüzyon yöntemi kullanılarak, elde edilen uçucu yağlarin Staphylacoccus aureus, Bacillus subtilis, Pseudomanas aeruginosa, Enterecoccus faecalis, Salmonella typhimurium ve Escherichia coli üzerindeki etkisi belirlenmiştir. Bunun için bakteri oluşumunun engellendiği bölgenin çapı ölçülmüştür. Bu test sonucunda JOO yapraklarından elde edilen uçucu yağın test edilen bütün mikroorganizmalara karşı etkili olduğu ve çevresinde bakteri üremesini engelleyen bir bölge oluşturduğu ancak engelleme etkisinin ampisilin ve ofloksin ile karşılaştırıldığında bu antibiyotikler kadar etkili olmadığı görülmüştür. J. oxycedrus L. subsp. oxycedrus yapraklarının uçucu yağları Escherichia coli’ye karşı 15 mm çapındaki engelleyici bölge ile en yüksek antibakteriyel etkiyi göstermiştir. En düşük antibakteriyel etkiyi ise 11 mm’lik bölge ile Staphylococcus aureus üzerinde göstermiştir. 

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Birincil Dil en
Konular Yaşam Bilimleri
Yayımlanma Tarihi Haziran 2018
Dergi Bölümü Makaleler
Yazarlar

Yazar: Neşe OKUT (Sorumlu Yazar)
Ülke: Turkey


Yazar: Bünyamin YILDIRIM
Ülke: Turkey


Yazar: Kamil EKİCİ
Ülke: Turkey


Yazar: Ömer TERZİOĞLU
Ülke: Turkey


Yazar: Fevzi ÖZGÖKÇE
Ülke: Turkey


Bibtex @araştırma makalesi { yyutbd379647, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, eissn = {1308-7584}, address = {Yüzüncü Yıl Üniversitesi}, year = {2018}, volume = {28}, pages = {186 - 191}, doi = {10.29133/yyutbd.379647}, title = {Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil}, key = {cite}, author = {TERZİOĞLU, Ömer and OKUT, Neşe and EKİCİ, Kamil and ÖZGÖKÇE, Fevzi and YILDIRIM, Bünyamin} }
APA OKUT, N , YILDIRIM, B , EKİCİ, K , TERZİOĞLU, Ö , ÖZGÖKÇE, F . (2018). Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 28 (2), 186-191. DOI: 10.29133/yyutbd.379647
MLA OKUT, N , YILDIRIM, B , EKİCİ, K , TERZİOĞLU, Ö , ÖZGÖKÇE, F . "Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 (2018): 186-191 <http://dergipark.gov.tr/yyutbd/issue/38909/379647>
Chicago OKUT, N , YILDIRIM, B , EKİCİ, K , TERZİOĞLU, Ö , ÖZGÖKÇE, F . "Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 (2018): 186-191
RIS TY - JOUR T1 - Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil AU - Neşe OKUT , Bünyamin YILDIRIM , Kamil EKİCİ , Ömer TERZİOĞLU , Fevzi ÖZGÖKÇE Y1 - 2018 PY - 2018 N1 - doi: 10.29133/yyutbd.379647 DO - 10.29133/yyutbd.379647 T2 - Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 186 EP - 191 VL - 28 IS - 2 SN - 1308-7576-1308-7584 M3 - doi: 10.29133/yyutbd.379647 UR - http://dx.doi.org/10.29133/yyutbd.379647 Y2 - 2018 ER -
EndNote %0 Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil %A Neşe OKUT , Bünyamin YILDIRIM , Kamil EKİCİ , Ömer TERZİOĞLU , Fevzi ÖZGÖKÇE %T Identification of Chemical Composition and Antibacterial Properties Juniperus oxycedrus L. subsp. oxycedrus Leaf Essential Oil %D 2018 %J Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi %P 1308-7576-1308-7584 %V 28 %N 2 %R doi: 10.29133/yyutbd.379647 %U 10.29133/yyutbd.379647
ISNAD OKUT, Neşe , YILDIRIM, Bünyamin , EKİCİ, Kamil , TERZİOĞLU, Ömer , ÖZGÖKÇE, Fevzi . "Juniperus oxycedrus L. subsp. oxycedrus Yapraklarındaki Uçucu Yağın Kimyasal Yapısı ve Antibakteriyel Özelliklerinin Belirlenmesi". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 28 / 2 (Haziran 2018): 186-191. http://dx.doi.org/10.29133/yyutbd.379647